Hematological and biochemical characterization of aging farm male rat strains in the National Center for Geriatrics and Gerontology.

Abstract

Laboratory rats, like mice, are a type of animal commonly used in scientific investigations as well as in basic aging and geriatric research. The selection of a rat strain is an important first step in the planning and design of an experiment due to physiological, anatomical, and ethological variations in each strain, which may significantly modify the expected results. In the present study, we characterized age-related changes, from 3 months old (mo) to 24 mo, in three male rat strains commonly used in medical research: RccHan^®️:WIST (RccHan:WIST), F344/NSlc (F344), and Slc:SD Rat (SD). The body weight, water/food consumption, and survival rate of each strain were physiologically evaluated. Hematological and biochemical values were analyzed every three months. Hematological results showed a decrease in lymphocytes and increases in other leukocytes from 12 mo in F344 and SD rats. The incidence of hematological disorder was 10-15% in F344 and SD rats from 18 mo. Increases in hepatic biochemical parameters (alanine transaminase (GPT/ALT) and aspartate transaminase (GOT/AST)) and cytopathological parameters (creatine phosphokinase (CPK)) were observed in male F344 rats at 12 mo. Triglycerides (TG) serum levels were significantly elevated in the 12 mo RccHan:WIST rats, while Lipase (LIP) levels were significantly reduced in 24 mo. The present results revealed significant variations in hematological and biochemical values in the different laboratory male rat strains due to genetic and nutritional-metabolic factors specific to each strain.