A combination of Citrus aurantifolia fruit rind and Theobroma cacao seed extracts supplementation enhances metabolic rates in overweight subjects: a randomized, placebo-controlled, cross-over study.

IF 3.5 4区医学 Q2 FOOD SCIENCE & TECHNOLOGY Food & Nutrition Research Pub Date : 2024-08-01 eCollection Date: 2024-01-01 DOI:10.29219/fnr.v68.10745

Nihal Kumar Reddy Ammatalli, Sesha Sai Siva Krishna Kuricheti, Sudipta Veeramachaneni, Yean Kyoung Koo, Guru Ramanathan, Amulya Yalamanchi

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Abstract

Background and objective: LN19183 is a proprietary, synergistic combination of Citrus aurantifolia fruit rind and Theobroma cacao seed extracts that increased resting energy expenditure (REE) in high-fat diet (HFD)-fed obese rats. The objective of this study was to validate the thermogenic potential of LN19183 in obese Sprague Dawley (SD) rats and to assess its clinical efficacy in a proof-of-concept, randomized, placebo-controlled, cross-over human trial.

Methods: In the rat study, HFD-fed obese rats were supplemented with either HFD alone or with 45, 90, or 180 mg LN19183 per kg body weight (BW) for 28 days. In the human study, 60 overweight adults (male and female, aged 20-39 years) were randomized. Subjects took LN19183 (450 mg) or a matched placebo capsule on two consecutive days in phases one and two of the study, separated by a 10-day washout period. In each phase, on day 1, REE at pre-dose, 60-, 120-, and 180-min post-dose, and on day 2, metabolic rates at pre-dose and post-dose during and 20 min after exercise were measured using indirect calorimetry.

Results: In rats, LN19183 significantly increased REE, reduced BW gain and fat masses, and increased fat and carbohydrate metabolism marker proteins including beta 3 adrenergic receptor (β3-AR), phospho-AMP-activated protein kinase (AMPK), glucagon-like peptide-1 receptor (GLP-1R) in the liver, and serum adiponectin levels. Furthermore, LN19183-supplemented human volunteers increased (P < 0.05, vs. placebo) the metabolic rates at rest and with exercise; their fat oxidation was increased (P < 0.05, vs. placebo) at rest and 20 min post-exercise. The groups' systolic and diastolic blood pressure (BP), heart rates (HR), and safety parameters were comparable.

Conclusion: These observations suggest that LN19183 is a thermogenic botanical composition with no stimulatory effects on BP and HR.

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一项随机、安慰剂对照、交叉研究：结合补充枳壳果皮和可可树籽提取物提高超重受试者的新陈代谢率。

背景和目的：LN19183 是柑橘果皮和可可豆籽提取物的专有协同组合，可增加高脂饮食（HFD）喂养的肥胖大鼠的静息能量消耗（REE）。本研究旨在验证 LN19183 在肥胖 Sprague Dawley（SD）大鼠中的生热潜力，并在一项概念验证、随机、安慰剂对照、交叉人体试验中评估其临床疗效：在大鼠研究中，每公斤体重（BW）的肥胖大鼠在连续 28 天的研究中分别补充高密度脂蛋白胆固醇（HFD）或 45、90 或 180 毫克 LN19183。在人体研究中，60 名超重成年人（男女均有，年龄在 20-39 岁之间）被随机分组。受试者在研究的第一和第二阶段连续两天服用 LN19183（450 毫克）或匹配的安慰剂胶囊，中间有 10 天的冲洗期。在每个阶段的第 1 天，使用间接热量计测量服药前、服药后 60 分钟、120 分钟和 180 分钟的 REE，以及第 2 天服药前和服药后运动中和运动后 20 分钟的代谢率：结果：LN19183能显著增加大鼠的REE，减少体重增加和脂肪量，增加脂肪和碳水化合物代谢标记蛋白，包括肝脏中的β3肾上腺素能受体（β3-AR）、磷酸-AMPK激活蛋白激酶（AMPK）、胰高血糖素样肽-1受体（GLP-1R）和血清脂肪连蛋白水平。此外，补充 LN19183 的人类志愿者在休息时和运动时的新陈代谢率都有所提高（与安慰剂相比，P < 0.05）；他们在休息时和运动后 20 分钟的脂肪氧化率也有所提高（与安慰剂相比，P < 0.05）。两组的收缩压和舒张压、心率和安全性参数相当：这些观察结果表明，LN19183 是一种生热植物成分，对血压和心率没有刺激作用。

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来源期刊

Food & Nutrition Research FOOD SCIENCE & TECHNOLOGY-NUTRITION & DIETETICS

CiteScore

5.20

自引率

9.10%

发文量

审稿时长

14 weeks

期刊介绍： Food & Nutrition Research is a peer-reviewed journal that presents the latest scientific research in various fields focusing on human nutrition. The journal publishes both quantitative and qualitative research papers. Through an Open Access publishing model, Food & Nutrition Research opens an important forum for researchers from academic and private arenas to exchange the latest results from research on human nutrition in a broad sense, both original papers and reviews, including: * Associations and effects of foods and nutrients on health * Dietary patterns and health * Molecular nutrition * Health claims on foods * Nutrition and cognitive functions * Nutritional effects of food composition and processing * Nutrition in developing countries * Animal and in vitro models with clear relevance for human nutrition * Nutrition and the Environment * Food and Nutrition Education * Nutrition and Economics Research papers on food chemistry (focus on chemical composition and analysis of foods) are generally not considered eligible, unless the results have a clear impact on human nutrition. The journal focuses on the different aspects of nutrition for people involved in nutrition research such as Dentists, Dieticians, Medical doctors, Nutritionists, Teachers, Journalists and Manufacturers in the food and pharmaceutical industries.