Predominance of DENV-3 among patients in Ouagadougou, Burkina Faso.

IF 0.8 4区医学 Q4 INFECTIOUS DISEASES Journal of Vector Borne Diseases Pub Date : 2024-07-07 DOI:10.4103/JVBD.JVBD_68_24

Abdoul Karim Ouattara, Shoukrat Ohuwa Toyin Bello, Alphonsine Ouédraogo, Lassina Traoré, Florencia Wendkuuni Djigma, Jacques Simporé

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Abstract

Background objectives: Dengue is an emerging vector-borne viral disease in tropical and subtropical areas such as Burkina Faso that experienced dengue outbreak in, 2013, 2016, 2017 and more recently in 2023. This study aimed to determine the seroprevalence and dengue serotype in suspected patients in Ouagadougou, Burkina Faso.

Methods: The study was conducted during October and November 2023 and included suspected febrile patients seen at HOSCO and CERBA. Plasma or serum samples were used for the detection of non-structural proteins (NS1) and IgM and IgG antibodies against the dengue virus using SD Bioline Dengue Duo rapid detection kit. Viral RNA was extracted using the QIAamp Viral RNA Mini Kit and dengue serotypes were determined by real-time RT-PCR using the Dengue Real-TM Genotype kit.

Results: The study population consisted of 896 patients, including 397 (44.3%) men and 499 (55.7%) women. Dengue seroprevalence was 16.5% (148/896) with 14.1% (126/896) of patients positive for the NS1 antigen, 1.3% (12/896) positive for IgM, and 2.7% (24/896) positive for IgG. Serotyping among 40 out of 45 positive patients revealed 77.5% (31/40) DENV-3, 17.5% (7/40) DENV-1, and 5.0% (2/40) DENV-2.

Interpretation conclusion: The present study report a high seroprevalence of dengue virus infection among patients during the months considered as the peak of infection in Burkina Faso. The results revealed a predominance of DENV-3. Continuous surveillance of dengue virus serotypes circulating in Burkina Faso is crucial.

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布基纳法索瓦加杜古的患者主要感染 DENV-3。

背景目标：在热带和亚热带地区，登革热是一种新出现的病媒传播病毒性疾病，例如布基纳法索曾在2013年、2016年、2017年和最近的2023年爆发过登革热疫情。本研究旨在确定布基纳法索瓦加杜古疑似患者的血清流行率和登革热血清型：研究于 2023 年 10 月和 11 月进行，包括在 HOSCO 和 CERBA 就诊的发热疑似患者。使用 SD Bioline Dengue Duo 快速检测试剂盒检测血浆或血清样本中的非结构蛋白（NS1）以及针对登革热病毒的 IgM 和 IgG 抗体。使用 QIAamp Viral RNA Mini Kit 提取病毒 RNA，并使用 Dengue Real-TM Genotype kit 通过实时 RT-PCR 测定登革热血清型：研究对象包括 896 名患者，其中男性 397 人（44.3%），女性 499 人（55.7%）。登革热血清阳性率为 16.5%（148/896），其中 14.1%（126/896）的患者 NS1 抗原阳性，1.3%（12/896）的患者 IgM 阳性，2.7%（24/896）的患者 IgG 阳性。45 名阳性患者中有 40 人的血清分型显示，77.5%（31/40）为 DENV-3，17.5%（7/40）为 DENV-1，5.0%（2/40）为 DENV-2：本研究报告显示，在布基纳法索登革热病毒感染高峰期的几个月中，患者的血清登革热病毒感染率很高。结果显示，登革病毒-3 型占主导地位。对布基纳法索流行的登革热病毒血清型进行持续监测至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vector Borne Diseases INFECTIOUS DISEASES-PARASITOLOGY

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： National Institute of Malaria Research on behalf of Indian Council of Medical Research (ICMR) publishes the Journal of Vector Borne Diseases. This Journal was earlier published as the Indian Journal of Malariology, a peer reviewed and open access biomedical journal in the field of vector borne diseases. The Journal publishes review articles, original research articles, short research communications, case reports of prime importance, letters to the editor in the field of vector borne diseases and their control.