A dual-signal mode electrochemical aptasensor based on tetrahedral DNA nanostructures for sensitive detection of citrinin in food using PtPdCo mesoporous nanozymes

IF 8.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2024-08-03 DOI:10.1016/j.foodchem.2024.140739
Shi Tang , Baoshan He , Yao Liu , Longdi Wang , Ying Liang , Jinshui Wang , Huali Jin , Min Wei , Wenjie Ren , Zhiguang Suo , Yiwei Xu
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Abstract

Citrinin (CIT) is a mycotoxin with nephrotoxicity and hepatotoxicity, presenting a significant threat to human health that is often overlooked. Therefore, a dual-signal mode (DPV and SWV) aptasensor for citrinin (CIT) detection was constructed based on tetrahedral DNA nanostructures (TDN) in this study. Furthermore, PtPdCo mesoporous nanozymes exhibit catalase-like catalytic functions, generating significant electrochemical signals through a Fenton-like reaction. Meanwhile their excellent Methylene Blue (MB) loading capability ensures independent dual signal outputs. The RecJf exonuclease-assisted (RecJf Exo-assisted) process can expand the linear detection range, enabling further amplification of the signal. Under optimized conditions, the constructed aptaensor exhibited excellent detection performance with limits of detection (LODs) of 7.67 × 10−3 ng·mL−1 (DPV mode) and 1.57 × 10−3 ng·mL−1 (SWV mode). Due to its multiple signal amplification and highly accurate dual-signal mode detection capability, this aptasensor shows promising potential for the in situ detection.

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基于四面体 DNA 纳米结构的双信号模式电化学诱导传感器,利用铂钯钴介孔纳米酶灵敏检测食品中的柠檬霉素。
橘皮霉素(CIT)是一种具有肾毒性和肝毒性的霉菌毒素,对人类健康构成重大威胁,但往往被忽视。因此,本研究基于四面体 DNA 纳米结构(TDN)构建了一种用于检测橘皮霉素(CIT)的双信号模式(DPV 和 SWV)灵敏传感器。此外,铂钯钴介孔纳米酶具有类似催化剂的催化功能,可通过类似芬顿的反应产生显著的电化学信号。同时,它们出色的亚甲基蓝(MB)负载能力确保了独立的双信号输出。RecJf 外切酶辅助(RecJf Exo-assisted)过程可以扩大线性检测范围,使信号进一步放大。在优化条件下,所构建的传感器表现出优异的检测性能,其检测限(LOD)为 7.67 × 10-3 ng-mL-1(DPV 模式)和 1.57 × 10-3 ng-mL-1(SWV 模式)。由于该传感器具有多重信号放大和高精度的双信号模式检测能力,因此在原位检测方面具有广阔的应用前景。
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文献相关原料
公司名称产品信息其他信息采购帮参考价格
上海源叶 cetyltrimethylammonium bromide
¥15.00~¥228861.16
上海源叶 bovine serum albumin
98%
¥27.00~¥90727.36
上海源叶 aflatoxin M1
¥900.00~¥36564.00
麦克林 Tris (2-carboxyethyl) phosphine and hydrochloride
98%
¥17.00~¥23079.59
阿拉丁 l-ascorbic acid
¥15.00~¥20675.84
阿拉丁 Chloroplatinic acid
99.95%
¥47.00~¥20472.12
上海源叶 deoxynivalenol
¥298.00~¥16380.00
上海源叶 aflatoxin G1
¥299.00~¥15570.00
上海源叶 6-mercapto-1-hexanol
98%
¥25.00~¥14337.00
上海源叶 Zearalenone
¥29.90~¥14000.00
阿拉丁 sodium palladium(II) tetrachloride
¥932.00~¥8484.00
上海源叶 Citrinin
¥470.00~¥8443.00
阿拉丁 cobalt chloride hexahydrate
¥15.00~¥7881.00
上海源叶 methylene blue
¥13.00~¥7749.00
来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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