Duddingtonia flagrans and its crude proteolytic extract: concomitant action in sheep coprocultures.

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Research Communications Pub Date : 2024-10-01 Epub Date: 2024-08-09 DOI:10.1007/s11259-024-10494-x
Debora Castro de Souza, Elias Honorato Gomes, Lisseth Bibiana Figueroa Puentes, Dásia Silveira Soares, Daniele Vieira da Silva, Laísa Bastos Albuquerque, Pedro Henrique Dutra Dos Santos, Tiago Moreira Facury, Fabio Ribeiro Braga, Filippe Elias de Freitas Soares
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Abstract

The presence of infective larvae (L3) of gastrointestinal nematode (GIN) parasites in pastures directly contributes to the constant recurrence of infections in ruminant herds. This study aimed to evaluate the nematophagous fungus Duddingtonia flagrans (AC001) (proteolytic crude extract and/or conidia) in the in vitro control of GIN L3 in coprocultures. To produce the proteolytic crude extract, a suspension (107 conidia/mL) of D. flagrans was inoculated into a liquid medium. After 6 days, the medium was filtered, centrifuged, and its proteolytic activity was measured. For the experimental assay, fecal samples were collected directly from the rectal ampulla of naturally infected sheep, and egg counts per gram of feces (EPG) were performed. Coprocultures were prepared using 10 g of fecal material with the groups defined as follows: control group G1 (1.0 mL of denatured proteolytic crude extract); treated group G2 (1.0 mL of active proteolytic crude extract); treated group G3 (1.0 mL of active proteolytic crude extract + 1.0 mL of AC001 conidia). The coprocultures were maintained at room temperature (25ºC), for 7 days, and then the L3 larvae were recovered. The results demonstrated that AC001 successfully produced protease (56.34 U/mL). The treatments with active proteolytic crude extract (G2) and active proteolytic crude extract + AC001 conidia (G3) were significantly different (p < 0.01) from the control group with denatured proteolytic crude extract (G1). AC001 and its proteolytic crude extract acted concomitantly on helminths directly in the fecal environment, suggesting potential future applications in the field.

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Duddingtonia flagrans 及其粗蛋白水解提取物:在绵羊共育中的协同作用。
牧场中胃肠道线虫(GIN)寄生虫感染性幼虫(L3)的存在直接导致了反刍动物群感染的不断复发。本研究旨在评估食线虫真菌 Duddingtonia flagrans (AC001)(蛋白水解粗提取物和/或分生孢子)在体外共培养中对 GIN L3 的控制作用。为了生产蛋白水解粗提取物,将 D. flagrans 的悬浮液(107 个分生孢子/毫升)接种到液体培养基中。6 天后,过滤培养基并离心,测量其蛋白水解活性。在实验测定中,直接从自然感染绵羊的直肠安瓿中收集粪便样本,并进行每克粪便中的虫卵计数(EPG)。使用 10 克粪便材料制备共培养物,各组定义如下:对照组 G1(1.0 毫升变性蛋白水解粗提取物);处理组 G2(1.0 毫升活性蛋白水解粗提取物);处理组 G3(1.0 毫升活性蛋白水解粗提取物 + 1.0 毫升 AC001 分生孢子)。共培养物在室温(25ºC)下保持 7 天,然后回收 L3 幼虫。结果表明,AC001 成功地产生了蛋白酶(56.34 U/mL)。活性蛋白水解粗提物处理(G2)和活性蛋白水解粗提物 + AC001 分生孢子处理(G3)有显著差异(p
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来源期刊
Veterinary Research Communications
Veterinary Research Communications 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
173
审稿时长
3 months
期刊介绍: Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
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