Effects of vitrification on mitochondrial ultrastructure and membrane potential and its distribution in mouse oocytes.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-09-01

C Zhang, M Zhao, Y Xue, X Tang, D Shi, X Wang

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Abstract

Background: Vitrification is commonly used for in vitro fertilization and has significant impact on gametes.

Objective: To investigate changes in ultrastructure, membrane potential and distribution of mitochondria in mouse oocytes after vitriﬁcation.

Materials and methods: Mouse oocytes were divided into three groups: one group as fresh control, one group for the toxicity test (treated with cryoprotectant but without vitrification), and the other for vitrification.

Results: Most mitochondria in oocytes were damaged after cooling and warming, being rough and fuzzy in appearance, even swollen and broken. The membrane potential of the toxicity test group and the vitrification group was 0.320 +/-0.030 and 0.244 +/- 0.038, respectively, in comparison to the fresh group (0.398 +/- 0.043). The membrane potential of the vitrified oocytes was significantly lower than fresh oocytes and the toxicity test oocytes (P % 0.05), but there was no significant difference between fresh oocytes and the toxicity test oocytes (P > 0.05). Mitochondria in fresh oocytes were denser and strained stronger, with 59.5> distributed homogeneously and 36.4> polarized. The majority of mitochondria in the toxicity-tested oocytes were clustered (69.3>) and only a small portion were distributed homogeneously (19.6>), while mitochondria in vitrified oocytes were clustered (56.3>) and deficient (24.4>), and their fluorescent staining was weak and blurred. There was a significant disruption in mitochondrial function after vitrification.

Conclusion: Vitrification alters the ultrastructure, membrane potential and distribution of mitochondria in oocytes, most likely caused by toxicity and mechanical injury. Doi.org/10.54680/fr24510110212.

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玻璃化对小鼠卵母细胞线粒体超微结构和膜电位及其分布的影响

背景：玻璃化常用于体外受精，对配子有重大影响：研究玻璃化后小鼠卵母细胞超微结构、膜电位和线粒体分布的变化：小鼠卵母细胞分为三组：一组为新鲜对照组，一组用于毒性试验（使用低温保护剂但未进行玻璃化处理），另一组用于玻璃化处理：结果：大多数卵母细胞中的线粒体在冷却和升温后受损，外观粗糙、模糊，甚至肿胀和破裂。毒性试验组和玻璃化组的膜电位分别为 0.320 +/-0.030 和 0.244 +/- 0.038，而新鲜组的膜电位为 0.398 +/- 0.043。玻璃化卵母细胞的膜电位明显低于新鲜卵母细胞和毒性试验卵母细胞（P % 0.05），但新鲜卵母细胞和毒性试验卵母细胞之间没有显著差异（P > 0.05）。新鲜卵母细胞中的线粒体密度更大，应变能力更强，59.5%分布均匀，36.4%呈极化分布。而玻璃化卵母细胞中的线粒体则呈簇状（56.3>）和缺失状（24.4>），且荧光染色弱而模糊。玻璃化后线粒体的功能受到严重破坏：结论：玻璃化改变了卵母细胞线粒体的超微结构、膜电位和分布，很可能是由毒性和机械损伤引起的。Doi.org/10.54680/fr24510110212.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.