Gastroprotective role of a flavonoid-rich subfraction from Fridericia chica (Bonpl.) L. G. Lohmann: a medicinal plant used in the Amazon region.

IF 4.6 2区医学 Q2 IMMUNOLOGY Inflammopharmacology Pub Date : 2024-10-01 Epub Date: 2024-08-10 DOI:10.1007/s10787-024-01544-6

Daniela Miorando, Amanda M Steffler, Cristian A Dalla Vecchia, Viviane L Simomura, Jaqueline J Veloso, Maike V Buzatto, Ruan K S Nunes, Lincon B Somensi, Max V Gutiérrez, Lorane I S H Melim, Franco M M Pontes, Luisa M Silva, Anzhela Veselinova, Lola González-Sánchez, Pablo G Jambrina, Walter A Roman Junior

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Abstract

Fridericia chica is an Amazonian plant used to treat stomach disorders. However, the pharmacological activity of flavonoids in the extract has yet to be investigated. Therefore, we considered that a flavonoid-rich F. chica subfraction (FRS) has gastroprotective functions. For this, before the induction of gastric ulcers with ethanol or piroxicam, the rats received vehicle (water), omeprazole (30 mg/kg), or FRS (30 mg/kg), and the ulcer area was measured macro and microscopically, and the antisecretory action was investigated in pylorus-ligated rats. In addition, the roles of nitric oxide (NO) and nonprotein sulfhydryl compounds (NP-SH) in the gastroprotective effects of FRS were studied. FRS reduced ethanol- and piroxicam-induced ulcerations by 81% and 77%, respectively, as confirmed histologically. Antioxidant effects were observed for FRS through the maintenance of GSH and LPO levels, and the SOD and CAT activity similar to those found in the nonulcerated group. Moreover, FRS avoided the increase in MPO activity and TNF, IL-6, IL-4 and IL-10 levels. Moreover, mucin staining increased in ulcerated rats receiving FRS, and the pharmacological mechanism gastroprotective seems to involve the NO and NP-SH in addition to antisecretory actions. The chemical study by mass spectrometry confirmed the presence of flavonoids in FRS, and molecular docking studies have shown that these compounds interact with cyclooxygenase-1 and NO synthase. Furthermore, there was no indication that FRS had cytotoxic effects. Our results support the popular use of F. chica, and we conclude that the gastroprotection effect promoted by FRS can be attributed to the combined effect of the flavonoids.

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亚马逊地区使用的一种药用植物 Fridericia chica (Bonpl.) L. G. Lohmann 中富含的黄酮类子萃取物具有保护胃黏膜的作用。

Fridericia chica 是一种亚马逊植物，可用于治疗胃病。然而，该提取物中黄酮类化合物的药理活性还有待研究。因此，我们认为富含黄酮类化合物的 F. chica 子馏分（FRS）具有胃保护功能。为此，在用乙醇或吡罗昔康诱导胃溃疡之前，给大鼠服用载体（水）、奥美拉唑（30 毫克/千克）或 FRS（30 毫克/千克），对溃疡面积进行宏观和显微测量，并对结扎幽门的大鼠进行抗分泌作用研究。此外，还研究了一氧化氮（NO）和非蛋白巯基化合物（NP-SH）在 FRS 胃保护作用中的作用。经组织学证实，FRS 可使乙醇和吡罗昔康诱发的溃疡分别减少 81% 和 77%。通过维持 GSH 和 LPO 水平，以及与非溃疡组相似的 SOD 和 CAT 活性，观察到 FRS 的抗氧化作用。此外，FRS 还避免了 MPO 活性和 TNF、IL-6、IL-4 和 IL-10 水平的升高。此外，接受 FRS 治疗的溃疡大鼠粘蛋白染色增加，胃保护的药理机制除了抗分泌作用外，似乎还涉及 NO 和 NP-SH。质谱化学研究证实，FRS 中含有黄酮类化合物，分子对接研究表明，这些化合物与环氧合酶-1 和 NO 合酶相互作用。此外，没有迹象表明 FRS 具有细胞毒性作用。我们的研究结果支持人们对 F. chica 的普遍使用，并得出结论认为，FRS 促进胃肠道保护的效果可归因于黄酮类化合物的综合作用。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]