Photopharmacological modulation of hippocampal local field potential by caged-glutamate with MicroLED probe.

IF 2 Q3 NEUROSCIENCES Neuropsychopharmacology Reports Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI:10.1002/npr2.12472

Shogo Okada, Noriaki Ohkawa, Kazuki Moriya, Yoshito Saitoh, Mikiko Ishikawa, Kakeru Oya, Atsushi Nishikawa, Hiroto Sekiguchi

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Abstract

Aim: Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation-dependent manner. Here, we investigated whether a microscale light-emitting diode (MicroLED) probe is applicable for the photoconversion of caged-glutamate (caged-Glu) in vivo.

Methods: A needle-shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged-Glu was infused into the DG and illuminated with light from a MicroLED probe.

Results: In the caged-Glu-injected DG, the LFP changed in the 10-20 Hz frequency ranges after light illumination, whereas there was no change in the ACSF control condition.

Conclusion: The MicroLED probe is applicable for photopharmacological experiments to modulate LFP with caged-Glu in vivo.

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用 MicroLED 探针对笼式谷氨酸海马局部场电位进行光药理学调节。

目的：光药理学是一种新技术，通过在精确时间内对特定目标区域的物质进行光转化来调节生物现象。笼状化合物被认为与光药理学兼容，因为非笼状配体会以依赖光照射的方式释放并发挥作用。在此，我们研究了微型发光二极管（MicroLED）探针是否适用于体内笼型谷氨酸（caged-Glu）的光化学转换：方法：制作了一个针形 MicroLED 探针，并将其插入小鼠海马齿状回（DG），探针上有一个用于注射药物的插管和一个用于测量局部场电位（LFP）的记录电极。将人工脑脊液（ACSF）或笼式-Glu注入DG，并用MicroLED探针照射：结果：在注入笼状 Glu 的 DG 中，光照后 LFP 在 10-20 Hz 频率范围内发生变化，而在 ACSF 对照条件下则没有变化：结论：MicroLED探针可用于光药理学实验，在体内用笼-Glu调节LFP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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