Immunohistochemical Profile of p62/SQSTM1/Sequestosome-1 in Human Low- and High-Grade Intracranial Meningiomas.

IF 2.6 4区 医学 Q3 CELL BIOLOGY Analytical Cellular Pathology Pub Date : 2024-08-02 eCollection Date: 2024-01-01 DOI:10.1155/2024/5573892
Antonio Ieni, Cristina Pizzimenti, Vincenzo Fiorentino, Mariausilia Franchina, Antonino Germanò, Giovanni Raffa, Maurizio Martini, Guido Fadda, Giovanni Tuccari
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Abstract

Among autophagic-related proteins, p62/SQSTM1/Sequestosome-1 represents a relevant actor in cellular proliferation and neoplastic growth. Although, recently, p62 expression has been analyzed in different neurodegenerative and glial neoplastic diseases, no available information have been reported in meningiomas, which have an high epidemiological relevance being the second most common category of intracranial tumors after gliomas. Generally meningiomas have a benign behavior, but their recurrence is not uncommon mainly when atypical or anaplastic varieties occur. However, intranuclear vacuoles have been ultrastructurally observed in meningiomas, and they were labelled by p62 antibodies. Therefore, in the present study, we have investigated p62 immunohistochemical pattern in a cohort of 133 cases representative of low- and high-grade meningiomas, to verify if p62 expression may be related to clinicopathological data, thus achieving a potential prognostic role. The p62 immunoexpression was frequently found in the nucleus and cytoplasm of neoplastic elements, and utilizing an intensity-distribution score, 55 (41.3%) cases were considered as high expressors while 78 (58.7%) cases were instead recorded as low expressors. Fifteen cases exhibited recurrences of the disease, 14 of which were codified as high expressors. Moreover, a direct relationship between p62 and Mib-1 immunoexpression as well as between p62 and neoplastic grade have been documented. Finally, we suggest that impaired autophagic flux with an increase in p62 expression may be involved in the activation of NRF2 also contributing in the development of recurrence in meningioma patients.

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人低度和高度颅内脑膜瘤中 p62/SQSTM1/Sequestosome-1 的免疫组化特征
在自噬相关蛋白中,p62/SQSTM1/Sequestosome-1 是细胞增殖和肿瘤生长的一个相关角色。虽然最近对不同神经退行性疾病和胶质肿瘤性疾病中 p62 的表达进行了分析,但还没有关于脑膜瘤中 p62 表达的报道,而脑膜瘤是仅次于胶质瘤的第二大类颅内肿瘤,具有很高的流行病学相关性。一般来说,脑膜瘤是良性的,但其复发并不少见,主要是在非典型或无弹性的情况下。然而,在脑膜瘤的超微结构中观察到核内空泡,并用 p62 抗体标记。因此,在本研究中,我们调查了一组 133 例具有代表性的低级别和高级别脑膜瘤的 p62 免疫组化模式,以验证 p62 的表达是否与临床病理数据相关,从而发挥潜在的预后作用。p62免疫表达经常出现在肿瘤细胞核和细胞质中,通过强度分布评分,55例(41.3%)被认为是高表达者,78例(58.7%)被认为是低表达者。有 15 个病例的病情出现复发,其中 14 个病例被定为高表达病例。此外,p62 和 Mib-1 免疫表达之间以及 p62 和肿瘤等级之间也有直接关系。最后,我们认为自噬通量受损和 p62 表达增加可能参与了 NRF2 的激活,这也是脑膜瘤患者复发的原因之一。
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来源期刊
Analytical Cellular Pathology
Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY
CiteScore
4.90
自引率
3.10%
发文量
70
审稿时长
16 weeks
期刊介绍: Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.
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