Overexpression of Extracellular Superoxide Dismutase 3 Inhibits Cancer Cell Growth and Migration in Colorectal Cancer.

IF 1.4 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Turkish Journal of Gastroenterology Pub Date : 2024-06-01 DOI:10.5152/tjg.2024.23232

Donghua Wang, Manyu Chen, Zhenggui Tao, Jinghu Du, Kui Tian, Zhen Chen, Bo Yu, Yu Chen, Long Lv

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Abstract

Background/aims: Incidence of colorectal cancer is rapidly increasing worldwide. Extracellular superoxide dismutase (EcSOD; SOD3) is an antioxidant enzyme. However, SOD3 roles in colorectal cancer progression remain uncertain.

Materials and methods: Superoxide dismutase 3 expression was evaluated, and we analyzed clinical relevance of SOD3 expression in colorectal cancer. Subsequently, SOD3 roles in colorectal cancer progression were detected by gain of function experiments. Changes in subcutaneous tumor and liver nodule size after SOD3 overexpression were examined in nude mice. The expression of proliferation marker Ki67 was assessed by immunohistochemical staining.

Results: Supperoxide dismutase 3 was downregulated in colorectal cancer (P <.01). Downregulation of SOD3 was correlated with unfavorable outcomes (P < .05). Superoxide dismutase 3 upregulation limited the proliferative (P <.05), migrative (P <.01) and invasive actions of colorectal cancer cells (P <.01) by suppressing epithelial-mesenchymal transition. Moreover, SOD3 overexpression reduced Ki67 expression (P <.01) and blocked tumor growth (P <01) and liver metastasis (P <.001) in mouse tumor model.

Conclusion: Superoxide dismutase 3 upregulation attenuates tumor growth and liver metastasis in colorectal cancer, suggesting that SOD3 has potential diagnostic and prognostic values regarding colorectal cancer treatment.

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过表达细胞外超氧化物歧化酶 3 可抑制结直肠癌癌细胞的生长和迁移

背景/目的：结直肠癌的发病率在全球范围内迅速上升。细胞外超氧化物歧化酶（EcSOD；SOD3）是一种抗氧化酶。然而，SOD3 在结直肠癌进展中的作用仍不确定：我们评估了超氧化物歧化酶 3 的表达，并分析了 SOD3 在结直肠癌中表达的临床意义。随后，通过功能增益实验检测了 SOD3 在结直肠癌进展中的作用。在裸鼠体内检测了 SOD3 过表达后皮下肿瘤和肝结节大小的变化。免疫组化染色评估了增殖标志物 Ki67 的表达：结果：超氧化物歧化酶 3 在结直肠癌中下调（P 结论：超氧化物歧化酶 3 在结直肠癌中上调：超氧化物歧化酶 3 的上调可减轻结直肠癌的肿瘤生长和肝转移，这表明 SOD3 对结直肠癌的治疗具有潜在的诊断和预后价值。

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来源期刊

Turkish Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

1.90

自引率

0.00%

发文量

127

审稿时长

6 months

期刊介绍： The Turkish Journal of Gastroenterology (Turk J Gastroenterol) is the double-blind peer-reviewed, open access, international publication organ of the Turkish Society of Gastroenterology. The journal is a bimonthly publication, published on January, March, May, July, September, November and its publication language is English. The Turkish Journal of Gastroenterology aims to publish international at the highest clinical and scientific level on original issues of gastroenterology and hepatology. The journal publishes original papers, review articles, case reports and letters to the editor on clinical and experimental gastroenterology and hepatology.