Curcumin-carbon dots suppress periodontitis via regulating METTL3/IRE1α signaling

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-08-01 DOI:10.1615/critreveukaryotgeneexpr.2024054463

Ling Li, Yueyan Wang, Lang Gao, Shunying Wu, Ying Jin

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Abstract

This study aimed to investigate the effects of curcumin-carbon dot conjugates (CUR-CD) on periodontitis. Porphyromonas gingivalis lipopolysaccharide (LPS) was used to establish periodontitis mode in vivo and in vitro. Histological analysis was conducted using HE staining. BMP2 and RUNX2 expression was determined using immunohistochemistry. mRNA levels were detected using RT-qPCR. Cytokine release was determined using ELISA assay. Osteogenic differentiation was detected using ALP staining. The results showed that CUR-CD inhibited the inflammatory response, as well as promoted bone healing in vivo and osteogenic differentiation in vitro. Moreover, CUR-CD downregulated METTL3, which inhibited m6A modification of IRE1α and downregulated IRE1α expression. However, overexpression of IRE1α reversed the effects of CUR-CD, stimulating inflammatory response and inhibiting bone healing and osteogenic differentiation. Collectively, CUR-CD inhibits the progression of periodontitis via downregulating IRE1α. Therefore, CUR-CD may be an alternative strategy for periodontitis.

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姜黄素碳点通过调节 METTL3/IRE1α 信号抑制牙周炎

本研究旨在探讨姜黄素-碳点共轭物（CUR-CD）对牙周炎的影响。研究使用牙龈卟啉菌脂多糖（LPS）在体内和体外建立牙周炎模式。采用 HE 染色法进行组织学分析。用免疫组化法测定 BMP2 和 RUNX2 的表达，用 RT-qPCR 法检测 mRNA 水平。使用酶联免疫吸附法测定细胞因子的释放。使用 ALP 染色法检测成骨分化。结果表明，CUR-CD 可抑制炎症反应，促进体内骨愈合和体外成骨分化。此外，CUR-CD还能下调METTL3，从而抑制IRE1α的m6A修饰并下调IRE1α的表达。然而，过量表达 IRE1α 会逆转 CUR-CD 的作用，刺激炎症反应并抑制骨愈合和成骨分化。总之，CUR-CD 可通过下调 IRE1α 抑制牙周炎的发展。因此，CUR-CD 可能是治疗牙周炎的另一种策略。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

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审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.