Tracking the metabolite footprint of four lactic acid bacteria in semiskimmed milk: A chemometric analysis

IF 2.5 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY International Journal of Dairy Technology Pub Date : 2024-08-06 DOI:10.1111/1471-0307.13127
Rodrigo Sigala‐Robles, María del Carmen Estrada‐Montoya, MarÍa J. Torres‐Llanez, Lourdes Santiago‐López, Adrián Hernández‐Mendoza, Belinda Vallejo‐Cordoba, Verónica Mata‐Haro, Abraham Wall‐Medrano, Aarón F. González‐Córdova
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Abstract

This study aimed to evaluate the strain‐specific fermentative behaviour and metabolite footprint of four Lactobacillus spp., in 48‐h fermented milks. Technological properties (net cell growth, pH and lactic acid production), exopolysaccharides (EPS), short‐chain fatty acids (SCFAs), α‐amino acids and peptides <3 kDa were determined and evaluated by chemometrics. Inter‐strain differences were attributed to technological properties, SCFAs and D‐mannose/galactose/arabinose production. Three clusters (technological properties, EPS and SCFA production) were confirmed by Pearson's correlation and hierarchical clustering and principal component analysis explained 61% of LAB's differential fermentative behaviour. Post hoc chemometric analysis is useful to select LAB with better post‐ and paraprobiotic potential.
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追踪半脂牛奶中四种乳酸菌的代谢足迹化学计量分析
本研究旨在评估四种乳酸杆菌在 48 小时发酵牛奶中的特定菌株发酵行为和代谢物足迹。采用化学计量学方法测定并评估了技术特性(细胞净生长、pH 值和乳酸产量)、外多糖(EPS)、短链脂肪酸(SCFA)、α-氨基酸和 3 kDa 肽。菌株间的差异归因于技术特性、SCFAs 和 D-甘露糖/半乳糖/阿拉伯糖的生产。通过皮尔逊相关性和分层聚类以及主成分分析,确认了三个聚类(技术特性、EPS 和 SCFA 产量),解释了 61% 的 LAB 发酵行为差异。事后化学计量分析有助于筛选出具有更好的后发酵潜力和副微生物潜力的 LAB。
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来源期刊
International Journal of Dairy Technology
International Journal of Dairy Technology 工程技术-食品科技
CiteScore
7.00
自引率
4.50%
发文量
76
审稿时长
12 months
期刊介绍: The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope. Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.
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