Metabolic Profile of Gut Microbiota and Levels of Trefoil Factors in Adults with Different Metabolic Phenotypes of Obesity

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Pub Date : 2024-08-07 DOI:10.1134/s0026893324700316

I. M. Kolesnikova, L. A. Ganenko, I. Yu. Vasilyev, T. V. Grigoryeva, N. I. Volkova, S. A. Roumiantsev, A. V. Shestopalov

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Abstract

Obesity is associated with changes in the gut microbiota, as well as with increased permeability of the intestinal wall. In 130 non-obese volunteers, 57 patients with metabolically healthy obesity (MHO), and 76 patients with metabolically unhealthy obesity (MUHO), bacterial DNA was isolated from stool samples, and the 16S rRNA gene was sequenced. The metabolic profile of the microbiota predicted by PICRUSt2 (https://huttenhower.sph.harvard.edu/picrust/) was more altered in patients with MUHO than MHO. Obesity, especially MUHO, was accompanied by an increase in the ability of the gut microbiota to degrade energy substrates, produce energy through oxidative phosphorylation, synthesize water-soluble vitamins (B1, B6, B7), nucleotides, heme, aromatic amino acids, and protective structural components of cells. Such changes may be a consequence of the microbiota adaptation to the MUHO-specific conditions. Thus, a vicious circle is formed, when MUHO promotes the depletion of the gut microbiome, and further degeneration of the latter contributes to the pathogenesis of metabolic disorders. The concentration of the trefoil factor family (TFF) in the serum of the participants was also determined. In MHO and MUHO patients, the TFF2 and TFF3 levels were increased, but we did not find significant associations of these changes with the metabolic profile of the gut microbiota.

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不同代谢表型肥胖成人的肠道微生物群代谢谱和三叶草因子水平

摘要肥胖与肠道微生物群的变化以及肠壁渗透性的增加有关。在 130 名非肥胖志愿者、57 名代谢健康型肥胖（MHO）患者和 76 名代谢不健康型肥胖（MUHO）患者中，从粪便样本中分离了细菌 DNA，并对 16S rRNA 基因进行了测序。根据 PICRUSt2（https://huttenhower.sph.harvard.edu/picrust/）预测，MUHO 患者的微生物群代谢特征比 MHO 患者有更大的改变。肥胖，尤其是 MUHO，伴随着肠道微生物群降解能量底物、通过氧化磷酸化产生能量、合成水溶性维生素（B1、B6、B7）、核苷酸、血红素、芳香族氨基酸和细胞保护结构成分能力的增强。这些变化可能是微生物群适应 MUHO 特定条件的结果。因此，当 MUHO 导致肠道微生物群枯竭时，就会形成恶性循环，而后者的进一步退化又会导致代谢紊乱的发病机制。此外，还测定了参与者血清中三叶草因子家族（TFF）的浓度。在 MHO 和 MUHO 患者中，TFF2 和 TFF3 的水平有所升高，但我们没有发现这些变化与肠道微生物群的代谢状况有明显的关联。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.