Membrane-anchored intracellular insulin receptor or insulin-like growth factor-1 receptor elicits ligand-independent downstream signaling

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-07-26 DOI:10.1016/j.bbrep.2024.101799
Akiko Sotozono , Kazuhiko Namekata , Xiaoli Guo , Youichi Shinozaki , Chikako Harada , Takahiko Noro , Tadashi Nakano , Takayuki Harada
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Abstract

Neurodegenerative diseases including glaucoma affect insulin signaling, and insulin treatment has been shown to reverse the neurodegenerative loss of dendritic complexity in retinal ganglion cells. Therefore, strategies for enhancing or maintaining insulin signaling are worth pursuing to establish new therapies for these diseases. In the present study, we generated constitutively active insulin receptor (F-iIR) and insulin-like growth factor-1 receptor (F-iIGF1R) using a system that forces membrane localization of the intracellular domains of these receptors by farnesylation. Immunohistochemistry and Western blot analysis revealed that F-iIR and F-iIGF1R caused the activation of ERK and AKT in the absence of ligands in vitro. Our results suggest that in vivo effects of F-iIR and F-iIGF1R on the progression of neurodegenerative diseases should be investigated in the future.

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膜锚定的细胞内胰岛素受体或胰岛素样生长因子-1 受体引发配体无关的下游信号传导
包括青光眼在内的神经退行性疾病会影响胰岛素信号传导,胰岛素治疗已被证明可逆转视网膜神经节细胞树突复杂性的神经退行性丧失。因此,增强或维持胰岛素信号转导的策略值得研究,以建立治疗这些疾病的新疗法。在本研究中,我们利用一种通过法尼基化迫使这些受体的胞内结构域膜定位的系统,生成了组成型活性胰岛素受体(F-iIR)和胰岛素样生长因子-1受体(F-iIGF1R)。免疫组化和 Western 印迹分析表明,F-iIR 和 F-iIGF1R 可在体外没有配体的情况下激活 ERK 和 AKT。我们的研究结果表明,未来应研究 F-iIR 和 F-iIGF1R 对神经退行性疾病进展的体内影响。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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