Whole genome sequence analysis of Bacillus amyloliquefaciens strain S2.5 as a potential probiotic for feed supplement in livestock production

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology Journal of Genetic Engineering and Biotechnology Pub Date : 2024-07-26 DOI:10.1016/j.jgeb.2024.100404
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Abstract

Background

Supplementing probiotics in livestock feed is increasing due to concerns over the potential harm caused by antibiotics and other chemical growth promoters. Several Bacillus sp. have been used as probiotic supplements for livestock. In this study, Bacillus amyloliquefaciens S2.5 was isolated from freshwater and its potential probiotic characteristics were evaluated in vitro. The whole genome of strain S2.5 was sequenced, and its probiotic traits were annotated using bioinformatic tools.

Results

Both vegetative cells and spores of strain S2.5 remained stable throughout the 1.5 h of gastric juice and 48 h of intestine simulation. The strain S2.5 harbored the ability to produce glucoamylase, carboxymethyl cellulase, protease, and chitinase. It is also susceptible to all six tested antibiotics. The complete genome sequence shows genes related to acid-bile tolerance, environmental stress resistance, hydrolases, and adhesion to gut mucosa, confirming probiotic traits in the in vitro experiments.

Conclusions

B. amyloliquefaciens S2.5 demonstrated potential probiotic characteristics and its genetic profile in the in vitro experiments. Further in vivo assessments of B. amyloliquefaciens S2.5 on livestock and poultry should be performed to assess its practical application.

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淀粉样芽孢杆菌 S2.5 菌株的全基因组序列分析--它可能是畜牧业饲料添加剂中的益生菌
背景由于人们担心抗生素和其他化学生长促进剂可能造成的危害,在牲畜饲料中添加益生菌的情况越来越多。一些芽孢杆菌已被用作家畜的益生菌补充剂。本研究从淡水中分离出淀粉芽孢杆菌 S2.5,并对其潜在的益生特性进行了体外评估。结果 S2.5 菌株的无性细胞和孢子在胃液模拟 1.5 小时和肠道模拟 48 小时内均保持稳定。菌株 S2.5 能够产生葡萄糖淀粉酶、羧甲基纤维素酶、蛋白酶和几丁质酶。它还对所有六种测试抗生素敏感。完整的基因组序列显示了与耐酸胆汁、抗环境压力、水解酶和粘附肠道粘膜有关的基因,证实了体外实验中的益生特性。应进一步评估淀粉样芽孢杆菌 S2.5 在家畜和家禽中的体内应用,以评估其实际应用情况。
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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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