Improved detection of mycobacteria in CF and tissue samples grown in mycobacteria growth indicator tube incubated at 30°C compared to conventional growth conditions of liquid and solid media.

IF 6.1 2区 医学 Q1 MICROBIOLOGY Journal of Clinical Microbiology Pub Date : 2024-09-11 Epub Date: 2024-08-13 DOI:10.1128/jcm.00683-24
J Kehrmann, A L Stumpf, A Dragaqina, J Buer
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Abstract

This study evaluates the growth of mycobacteria in samples from cystic fibrosis (CF) patients and tissue samples using the mycobacteria growth indicator tube (MGIT) incubated at 30°C in comparison to conventional MGIT cultures incubated at 37°C in a BACTEC MGIT 960 device and solid media incubated at 36°C and 30°C. A total of 1,549 samples were analyzed, of which 202 mycobacterial isolates were cultured from 197 positive specimens, including five mixed cultures. The highest detection rate was achieved from MGIT at 30°C, with 84.2% of mycobacterial isolates (170 of 202), which was significantly higher than any other culture condition (P < 0.0001 for any condition). MGIT at 37°C yielded 61.4% (124 of 202) of the recovered isolates, whereas Löwenstein Jensen (LJ) and Stonebrink at 36°C, and LJ and Stonebrink at 30°C retrieved 47.0% (95), 49.5% (100), 50.0% (101), and 53.0% (107) of the isolates, respectively. Of the 53 isolates that were grown exclusively under one culture condition, the highest number of isolates (36) was recovered from MGIT incubated at 30°C. MGIT at 37°C recovered eight of the 53 isolates, whereas LJ incubated at 30°C and Stonebrink incubated at 30°C and 36°C recovered five, three, and one isolate, respectively. No isolates were grown exclusively from LJ incubated at 36°C. In CF patients and tissue samples, MGIT cultivated at 30°C for 8 weeks increases the performance of mycobacterial culture.

Importance: Our study shows that the addition of mycobacteria growth indicator tube (MGIT) liquid culture incubated at 30°C improves the detection of mycobacteria from CF and tissue samples. MGIT incubated at 30°C recovered significantly more mycobacterial isolates than MGIT incubated at 37°C and significantly more isolates than either Lowenstein Jensen or Stonebrink solid media incubated at either 36°C or 30°C. Of 202 mycobacterial isolates recovered from 1,549 specimens, 170 were recovered from MGIT incubated at 30°C, followed by MGIT incubated at 37°C with 124 isolates and solid media culture conditions that recovered between 95 and 107 mycobacterial isolates. All conventional culture conditions combined without MGIT incubated at 30°C recovered 166 isolates. MGIT incubated at 30°C recovered the highest number of isolates detected exclusively by a single culture condition and recovered mycobacterial isolates of highly relevant mycobacterial species, including Mycobacterium abscessus and Mycobacterium tuberculosis.

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与传统的液体和固体培养基生长条件相比,在 30°C 下培养的分枝杆菌生长指示管中生长的 CF 和组织样本中分枝杆菌的检测能力更强。
本研究评估了囊性纤维化(CF)患者样本和组织样本中分枝杆菌的生长情况,使用的分枝杆菌生长指示管(MGIT)在 30°C 下培养,与 BACTEC MGIT 960 设备中 37°C 下培养的传统 MGIT 培养物以及 36°C 和 30°C 下培养的固体培养基进行了比较。共分析了 1,549 份样本,其中从 197 份阳性样本(包括 5 份混合培养物)中培养出 202 株分枝杆菌。30°C 下的 MGIT 检出率最高,达到 84.2%(202 个样本中的 170 个),明显高于其他任何培养条件(任何条件下的 P 均小于 0.0001)。37°C 下的 MGIT 得到了 61.4% 的分离物(202 个中的 124 个),而 36°C 下的 Löwenstein Jensen(LJ)和 Stonebrink 以及 30°C 下的 LJ 和 Stonebrink 则分别得到了 47.0% (95 个)、49.5% (100 个)、50.0% (101 个)和 53.0% (107 个)的分离物。在只在一种培养条件下生长的 53 个分离物中,从 30°C 培养的 MGIT 中回收的分离物数量最多(36 个)。在 37 摄氏度条件下培养的 MGIT 在 53 个分离物中回收了 8 个,而在 30 摄氏度条件下培养的 LJ 以及在 30 摄氏度和 36 摄氏度条件下培养的 Stonebrink 分别回收了 5 个、3 个和 1 个分离物。在 36°C 培养的 LJ 中没有生长出纯粹的分离物。在 CF 患者和组织样本中,30°C 培养 8 周的 MGIT 可提高分枝杆菌的培养效果:我们的研究表明,加入 30°C 培养的分枝杆菌生长指示管(MGIT)液体培养液可提高从 CF 和组织样本中检测分枝杆菌的能力。在 30°C 下培养的 MGIT 比在 37°C 下培养的 MGIT 能回收更多的分枝杆菌,也比在 36°C 或 30°C 下培养的 Lowenstein Jensen 或 Stonebrink 固体培养基能回收更多的分枝杆菌。在从 1,549 份标本中回收的 202 株分枝杆菌中,有 170 株是从 30°C 培养的 MGIT 中回收的,其次是 37°C 培养的 MGIT,回收了 124 株分枝杆菌,而固体培养基培养条件则回收了 95 至 107 株分枝杆菌。所有常规培养条件加在一起,但不包括 30°C 培养的 MGIT,共培养出 166 个分离株。在 30°C 下培养的 MGIT 在单一培养条件下回收的分离株数量最多,而且回收的分枝杆菌属于高度相关的分枝杆菌种类,包括脓肿分枝杆菌和结核分枝杆菌。
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来源期刊
Journal of Clinical Microbiology
Journal of Clinical Microbiology 医学-微生物学
CiteScore
17.10
自引率
4.30%
发文量
347
审稿时长
3 months
期刊介绍: The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.
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