Exploration and genetic analyses of canopy leaf pigmentation changes in soybean (Glycine max L.): unveiling a novel phenotype.

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-08-13 DOI:10.1007/s00122-024-04693-y
Hee Jin You, Hyun Jo, Ji-Min Kim, Sung-Taeg Kang, Ngoc Ha Luong, Yeong-Ho Kim, Sungwoo Lee
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Abstract

Key message: Pigmentation changes in canopy leaves were first reported, and subsequent genetic analyses identified a major QTL associated with levels of pigmentation changes, suggesting Glyma.06G202300 as a candidate gene. An unexpected reddish-purple pigmentation in upper canopy leaves was discovered during the late reproductive stages in soybean (Glycine max L.) genotypes. Two sensitive genotypes, 'Uram' and PI 96983, exhibited anomalous canopy leaf pigmentation changes (CLPC), while 'Daepung' did not. The objectives of this study were to: (i) characterize the physiological features of pigmented canopy leaves compared with non-pigmented leaves, (ii) evaluate phenotypic variation in a combined recombinant inbred line (RIL) population (N = 169 RILs) under field conditions, and (iii) genetically identify quantitative trait loci (QTL) for CLPC via joint population linkage analysis. Comparison between pigmented and normal leaves revealed different Fv/Fm of photosystem II, hyperspectral reflectance, and cellular properties, suggesting the pigmentation changes occur in response to an undefined abiotic stress. A highly significant QTL was identified on chromosome 6, explaining ~ 62.8% of phenotypic variance. Based on the QTL result, Glyma.06G202300 encoding flavonoid 3'-hydroxylase (F3'H) was identified as a candidate gene. In both Uram and PI 96983, a 1-bp deletion was confirmed in the third exon of Glyma.06G202300 that results in a premature stop codon in both Uram and PI 96983 and a truncated F3'H protein lacking important domains. Additionally, gene expression analyses uncovered significant differences between pigmented and non-pigmented leaves. This is the first report of a novel symptom and an associated major QTL. These results will provide soybean geneticists and breeders with valuable knowledge regarding physiological changes that may affect soybean production. Further studies are required to elucidate the causal environmental stress and the underlying molecular mechanisms.

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大豆(Glycine max L.)冠层叶片色素变化的探索和遗传分析:揭示一种新的表型。
关键信息:冠层叶片的色素变化首次被报道,随后的遗传分析发现了一个与色素变化水平相关的主要 QTL,表明 Glyma.06G202300 是一个候选基因。在大豆(Glycine max L.)基因型的生殖后期,发现冠层上部叶片出现了意想不到的紫红色色素沉着。两个敏感基因型'Uram'和 PI 96983 表现出异常冠层叶片色素变化(CLPC),而'Daepung'则没有。本研究的目的是(i) 与非色素斑叶片相比,描述色素斑冠层叶片的生理特征;(ii) 评估田间条件下重组近交系(RIL)群体(N = 169 RILs)的表型变异;(iii) 通过群体联合连锁分析,从遗传学上鉴定 CLPC 的数量性状位点(QTL)。色素沉着叶片与正常叶片的比较显示了不同的光系统 II Fv/Fm、高光谱反射率和细胞特性,表明色素沉着变化是对未确定的非生物胁迫的响应。在 6 号染色体上发现了一个高度显著的 QTL,解释了约 62.8% 的表型变异。根据 QTL 结果,编码黄酮类化合物 3'-羟化酶(F3'H)的 Glyma.06G202300 被确定为候选基因。在 Uram 和 PI 96983 中,Glyma.06G202300 的第三个外显子上都有一个 1-bp 的缺失,这导致 Uram 和 PI 96983 中都有一个过早的终止密码子,F3'H 蛋白被截短,缺乏重要的结构域。此外,基因表达分析发现色素叶片和非色素叶片之间存在显著差异。这是首次报道一种新的症状和相关的主要 QTL。这些结果将为大豆遗传学家和育种家提供有关可能影响大豆产量的生理变化的宝贵知识。需要进一步的研究来阐明致病的环境胁迫和潜在的分子机制。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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