Expression, purification, and function of baculovirus infected insect cells-derived hookworm AIP-1 and AIP-2 proteins

IF 1.2 4区 农林科学 Q3 ENTOMOLOGY Entomological Research Pub Date : 2024-08-12 DOI:10.1111/1748-5967.12760
Chae Eun Lee, Hyun Joo Chung, Da Won Lee, Jong Seok Lim, Jeong Hwan Lee, Kisung Ko, Soon Auck Hong, Min Eui Hong, Joo Young Kim, Hye Jun Lee, Jin Wook Kim, Soon Chul Myung
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Abstract

Anti-inflammatory protein (AIP)-1 and AIP-2, identified as parasite excretory/secretory (ES) proteins, play a crucial role in promoting the survival of the parasite and evading the host immunological response. Both proteins inhibit inflammatory reactions, induce apoptosis in effector cells, and influence the phenotype of the immune response. Numerous parasite-derived proteins have shown promise as therapeutic targets for inflammatory and allergic diseases. Despite this, the precise biological roles, and molecular characteristics of many such proteins remain unclear.

In this study, AIP-1 and AIP-2 were produced in the baculovirus-insect cell expression system (BEVS). The multiplicity of infection (MOI) and the duration of infection conditions for AIP-1 and AIP-2 protein expression were successfully optimized to induce the highest expression levels of AIP-1 and AIP-2 proteins in insect cells. The insect cell-derived AIP-1 and AIP-2 exhibited inhibitory functions against human matrix metalloproteinases (MMPs). This study demonstrates that insect cell-derived AIP-1 and AIP-2 have the potential as therapeutic proteins for MMP-TIMP (Metalloprotease-Tissue inhibitor of metalloprotease) axis related disease.

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杆状病毒感染昆虫细胞衍生的钩虫 AIP-1 和 AIP-2 蛋白的表达、纯化和功能
抗炎蛋白(AIP)-1 和 AIP-2 被确认为寄生虫的排泄/分泌(ES)蛋白,在促进寄生虫生存和逃避宿主免疫反应方面发挥着至关重要的作用。这两种蛋白都能抑制炎症反应,诱导效应细胞凋亡,并影响免疫反应的表型。许多寄生虫衍生蛋白有望成为炎症和过敏性疾病的治疗靶点。在这项研究中,AIP-1 和 AIP-2 是在杆状病毒-昆虫细胞表达系统(BEVS)中产生的。本研究在杆状病毒-昆虫细胞表达系统(BEVS)中制备了AIP-1和AIP-2,成功优化了AIP-1和AIP-2蛋白表达的感染倍数(MOI)和感染持续时间,以诱导昆虫细胞中AIP-1和AIP-2蛋白的最高表达水平。昆虫细胞衍生的 AIP-1 和 AIP-2 对人类基质金属蛋白酶(MMPs)具有抑制作用。这项研究表明,昆虫细胞衍生的 AIP-1 和 AIP-2 蛋白具有治疗 MMP-TIMP(金属蛋白酶-组织金属蛋白酶抑制剂)轴相关疾病的潜力。
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来源期刊
CiteScore
2.50
自引率
7.70%
发文量
64
期刊介绍: Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered: -systematics- ecology- physiology- biochemistry- pest control- embryology- genetics- cell and molecular biology- medical entomology- apiculture and sericulture. The Journal publishes research papers and invited reviews.
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