Chien-Yu Huang, Chong-You Chen, Chia-Hung Wei, Jia-Wei Yang, Yu-Chien Lin, Chih-Fei Kao, Johnson H. Y. Chung and Guan-Yu Chen
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引用次数: 0
Abstract
Graphene oxide (GO) is a two-dimensional metastable nanomaterial. Interestingly, GO formed oxygen clusterings in addition to oxidized and graphitic phases during the low-temperature thermal annealing process, which could be further used for biomolecule bonding. By harnessing this property of GO, we created a bio-interface with patterned structures with a common laboratory hot plate that could tune cellular behavior by physical contact. Due to the regional distribution of oxygen clustering at the interface, we refer to it as patterned annealed graphene oxide (paGO). In addition, since the paGO was a heterogeneous interface and bonded biomolecules to varying degrees, arginine–glycine–aspartic acid (RGD) was modified on it and successfully regulated cellular-directed growth and migration. Finally, we investigated the FRET phenomenon of this heterogeneous interface and found that it has potential as a biosensor. The paGO interface has the advantages of easy regulation and fabrication, and the one-step thermal reduction method is suitable for biological applications. We believe that this low-temperature thermal annealing method would make GO interfaces more accessible, especially for the development of nano-interfacial modifications for biological applications, revealing its potential for biomedical applications.
氧化石墨烯(GO)是一种二维可迁移纳米材料。有趣的是,在低温热退火过程中,除了氧化相和石墨相之外,GO 还形成了氧簇,可进一步用于生物分子键合。利用 GO 的这一特性,我们用实验室中常见的热板制造出了具有图案化结构的生物界面,可以通过物理接触调整细胞行为。由于氧在界面上呈区域性分布,我们将其称为图案化退火氧化石墨烯(paGO)。此外,由于 paGO 是一种异质界面,可不同程度地结合生物大分子,精氨酸-甘氨酸-天冬氨酸(RGD)被修饰在其上,并成功地调节了细胞定向生长和迁移。最后,我们研究了这种异质界面的 FRET 现象,发现它具有作为生物传感器的潜力。paGO 界面具有易于调节和制造的优点,而且一步热还原法适合生物应用。我们相信,这种低温热退火方法将使 GO 界面更容易获得,特别是在开发用于生物应用的纳米界面修饰方面,揭示了其在生物医学方面的应用潜力。
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices