Downregulation of RNF128 Inhibits the Proliferation, Migration, Invasion and EMT of Colorectal Cancer Cells.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Genetics Pub Date : 2024-08-15 DOI:10.1007/s10528-024-10895-2

Meng Wang, Jian Ding, Aihong Zhao, Yixin Zhang, Yongkun Zhou, Zhaochun Tian

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Abstract

Colorectal cancer has the third highest incidence and second highest mortality rate among all cancer types. Exploring the molecular mechanisms driving malignant proliferation and metastasis of colorectal cancer will benefit the treatment and management of cancer patients. Recent studies have reported diametrically opposed roles of Ring finger protein 128 (RNF128) in different types of cancer. However, the role of RNF128 in colorectal cancer is still completely unknown, which this study attempts to analyze. The differential expression of RNF128 mRNA and protein in 30 pairs of colorectal cancer and corresponding peritumoral tissues was detected using RT-qPCR, western blot and immunohistochemical staining. siRNA specifically targeting RNF128 was transfected into colorectal cancer cell lines (SW1116 and SW480) cultured in vitro. Proliferation, growth, migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer cells were examined by CCK-8, clone formation, wound-healing, transwell, western blot and immunofluorescence assays. Both RNF128 mRNA and protein levels were significantly increased in colorectal cancer tissues compared to pericarcinoma tissues. Knockdown of RNF128 significantly inhibited the proliferation, growth, migration, invasion and EMT of SW480 and SW1116 cells. Targeting RNF128 may benefit the treatment and management of colorectal cancer.

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下调 RNF128 可抑制结直肠癌细胞的增殖、迁移、侵袭和 EMT。

在所有癌症类型中，结直肠癌的发病率位居第三，死亡率位居第二。探索驱动结直肠癌恶性增殖和转移的分子机制将有利于癌症患者的治疗和管理。最近有研究报告称，环指蛋白 128（RNF128）在不同类型癌症中的作用截然相反。然而，RNF128 在结直肠癌中的作用仍然完全未知，本研究试图对此进行分析。本研究采用 RT-qPCR、Western 印迹和免疫组化染色法检测了 30 对结直肠癌和相应瘤周组织中 RNF128 mRNA 和蛋白的差异表达。通过 CCK-8、克隆形成、伤口愈合、Transwell、Western 印迹和免疫荧光试验检测了结直肠癌细胞的增殖、生长、迁移、侵袭和上皮-间质转化（EMT）。与周围癌组织相比，结直肠癌组织中的 RNF128 mRNA 和蛋白水平均明显升高。敲除 RNF128 能明显抑制 SW480 和 SW1116 细胞的增殖、生长、迁移、侵袭和 EMT。以RNF128为靶点可能有益于结直肠癌的治疗和管理。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.