Magnolin alleviates cyclophosphamide-induced oxidative stress, inflammation, and apoptosis via Nrf2/HO-1 signaling pathway.

IF 2.2 4区 医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-08-14 eCollection Date: 2024-08-01 DOI:10.1093/toxres/tfae129
Sinan Ince, Hasan Huseyin Demirel, Ezgi Nur Demirkapi, Ismail Kucukkurt, Abdullah Eryavuz, Damla Arslan-Acaroz, Ulas Acaroz, Ali Tureyen
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Abstract

In the present study, we investigated the protective effect of magnolin (MAG) against oxidative stress induced by cyclophosphamide (CP) and its role in the Nrf2/HO-1 signaling pathway. Rats were administered MAG (1 mg/kg, i.p.) for 14 days and CP (75 mg/kg, i.p.) on the 14th day. CP administration increased tissue damage, as evidenced by elevated levels of transaminases (aspartate and alanine), alkaline phosphatase, and renal parameters (blood urea nitrogen and creatinine). Additionally, 8-hydroxy-2'-deoxyguanosine and malondialdehyde levels were increased, whereas glutathione levels, along with catalase and superoxide dismutase activities, decreased in CP-treated rats. CP also down-regulated the expression of Bcl-2, HO-1, Nrf2, and NQO-1, while up-regulating Bax, Cas-3, TNF-α, Cox-2, iNOS, IL-6, IL-1β, and NFκB in liver and kidney tissues. In addition, CP treatment caused histopathological changes in heart, lung, liver, kidney, brain, and testis tissues. Treatment with MAG improved biochemical and oxidative stress parameters and prevented histopathological changes in CP-treated rats. Moreover, MAG suppressed the expression of inflammatory cytokines and apoptosis markers. In conclusion, MAG effectively prevented CP-induced toxicity by reducing oxidative stress, inflammation, and apoptosis, with its protective efficacy associated with the up-regulation of Nrf2/HO-1 signaling.

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木兰脂素可通过Nrf2/HO-1信号通路减轻环磷酰胺诱导的氧化应激、炎症和细胞凋亡。
在本研究中,我们探讨了木兰脂素(MAG)对环磷酰胺(CP)诱导的氧化应激的保护作用及其在Nrf2/HO-1信号通路中的作用。大鼠连续14天服用MAG(1毫克/千克,静注),第14天服用CP(75毫克/千克,静注)。氯化石蜡会增加组织损伤,表现为转氨酶(天门冬氨酸和丙氨酸)、碱性磷酸酶和肾参数(血尿素氮和肌酐)水平升高。此外,CP 处理的大鼠体内 8- 羟基-2'-脱氧鸟苷和丙二醛水平升高,而谷胱甘肽水平以及过氧化氢酶和超氧化物歧化酶活性降低。CP 还下调肝脏和肾脏组织中 Bcl-2、HO-1、Nrf2 和 NQO-1 的表达,同时上调 Bax、Cas-3、TNF-α、Cox-2、iNOS、IL-6、IL-1β 和 NFκB 的表达。此外,氯化石蜡还导致心、肺、肝、肾、脑和睾丸组织发生组织病理学变化。用 MAG 治疗可改善 CP 处理大鼠的生化和氧化应激参数,并防止组织病理学变化。此外,MAG 还能抑制炎症细胞因子和细胞凋亡标志物的表达。总之,MAG 通过降低氧化应激、炎症和细胞凋亡,有效预防了 CP 诱导的毒性,其保护功效与 Nrf2/HO-1 信号的上调有关。
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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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