Insecticidal and Repellent Activity of Essential Oils from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea Against Immature and Adult Stages of Ctenocephalides felis felis

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2024-08-15 DOI:10.1007/s11686-024-00874-3

Emily Andressa Santos Lima, Diefrey Ribeiro Campos, Eduardo Fellipe Melo Santos Soares, Anna Beatriz Ribeiro Fortunato, Taynara Monsores e Silva, Nayana de Figueiredo Pereira, Douglas Siqueira de Almeida Chaves, Yara Peluso Cid, Katherina Coumendouros

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Abstract

Purpose

The flea Ctenocephalides felis (Siphonaptera: Pulicidae), parasitizes dogs and cats globally, acting as a vector for various pathogens affecting both animals and humans. Growing interest in environmentally friendly, plant-based products prompted this study. The aim of the study was to determine the chemical composition of essential oils (EOs) from Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, assessing their insecticidal and repellent properties, determining lethal concentrations (LC50 and LC90), and evaluating residual efficacy in vitro against Ctenocephalides felis felis.

Methods

Gas Chromatography with Flame Ionization Detector analyzed EO composition. In vitro tests involved preparing EO solutions at various concentrations. Ten specimens from each life stage (egg, larva, pupa, adult) were used for insecticidal activity assessment. Adulticidal activity was assessed using 10 cm² filter paper strip, each treated with 0.200 mL of the test solution. Immature stages activities were evaluated using 23.76 cm² discs of the same filter paper, each treated with 0.470 mL of the test solution. Mortality percentage was calculated using (number of dead insects × 100) / number of incubated insects. Probit analysis calculated LC50 values with a 95% confidence interval.

Results

Major EO constituents were β-caryophyllene (EOCR), linalool (EOLH), linalyl acetate (EOSS), and limonene (EOCP). LC50 values were obtained for all stages except for the essential oil of C. paradisi. All oils showed repellent activity at 800 μg/cm². OECR exhibited greater residual efficacy.

Conclusion

Each EO demonstrated superior insecticidal activity against specific C. felis felis stages.

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Copaifera reticulata、Citrus paradisi、Lavandula hybrida 和 Salvia sclarea 的精油对栉水母幼虫和成虫的杀虫和驱虫活性。

目的：跳蚤栉头蚤（Siphonaptera：Pulicidae）在全球范围内寄生于狗和猫，是影响动物和人类的各种病原体的媒介。人们对环境友好型植物产品的兴趣与日俱增，促使我们开展了这项研究。本研究的目的是确定 Copaifera reticulata、Citrus paradisi、Lavandula hybrida 和 Salvia sclarea 的精油 (EOs) 的化学成分，评估其杀虫和驱虫特性，确定致死浓度（LC50 和 LC90），并在体外评估对猫栉水母的残留功效：方法：采用火焰离子化检测器气相色谱法分析环氧乙烷的成分。体外测试包括制备不同浓度的环氧乙烷溶液。每个生命阶段（卵、幼虫、蛹、成虫）的 10 个标本用于杀虫活性评估。成虫活性用 10 平方厘米的滤纸条进行评估，每条滤纸条用 0.200 毫升的试验溶液处理。未成熟阶段的活性使用 23.76 平方厘米的相同滤纸圆盘进行评估，每个圆盘用 0.470 毫升试验溶液处理。死亡率用（死亡昆虫数 × 100）/孵化昆虫数计算。Probit 分析计算 LC50 值，置信区间为 95%：结果：主要环氧乙烷成分为 β-石竹烯（EOCR）、芳樟醇（EOLH）、乙酸芳樟酯（EOSS）和柠檬烯（EOCP）。除 C. paradisi 的精油外，所有阶段都获得了 LC50 值。在 800 μg/cm2 的浓度下，所有精油都具有驱避活性。结论：结论：每种环氧乙烷都显示出对特定阶段的猫科动物具有卓越的杀虫活性。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.