Molecular survey of certain protozoan agents that cause diarrhea in children in Sudan.

Q2 Pharmacology, Toxicology and Pharmaceutics F1000Research Pub Date : 2024-10-23 eCollection Date: 2022-01-01 DOI:10.12688/f1000research.123652.3

Mosab Adam, Hongwei Shen, Khalid-A Enan, Hao Wang, Azza B Musa Musa, Abdel R El Hussein, Isam M Khidir, Xuejun Ma

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Abstract

Introduction: Diarrhea is a significant health problem in the Third World. Identification of the pathogen that causes diarrhea is vital for measures to prevent and control this disease. There are also very few reports of diarrhea in Sudan. Our study aimed to determine the Prevalence of specific protozoan pathogens ( Entamoeba histolytica, Cryptosporidium parvum., and Giardia spp) in children in Khartoum, Sudan.

Methods: We conducted a cross-sectional survey among children under five years of age hospitalized with acute diarrhea between April and December 2014. Diarrheal stool samples were collected, and E. histolytica, C. parvum, and Giardia spp were examined using multiplex real-time PCR.

Results: Four hundred and thirty-seven children with acute diarrhea were included in this study; the higher prevalence of diarrhea was in the age ≤ 2 years old (403, 92.2%), >2-≤4 years (32, 7.3%), and >4-<5 years (2, 0.5%). The male-to-female ratio in this study was 1:1.7. Infection with intestinal parasite was found in 155 (35.5%) cases, and co-infection was detected in 16 (3.7%) cases. Giardia spp (18.8%) and C. parvum (15.8%) were the most frequently identified parasites, followed by E. histolytica (0.9). The parasite infection rate was highest and lowest in the under 2-year-old group 143 (35.5%) and the 2-4-year-old group 12 (37.5%). The infection rate was higher in boys 104 (37.7%) than in girls 51 (31.7%). The number of positive cases was higher in the rainy season (August to December) 143 (37.4%), corresponding with that in the dry Season (April to June) 12 (21.8%).

Discussion: Our present study demonstrated the high prevalence of Giardia spp and C. parvum in children with diarrhea in the Khartoum region and the usefulness of the multiplex real-time method in disclosing pathogenic protozoal agents. Our result highlighted the necessity of developing intervention measurement and control strategies to deal with childhood parasitic diarrhea in this region.

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对引起苏丹儿童腹泻的某些原生动物病原体进行分子调查。

导言腹泻是第三世界的一个重大健康问题。确定导致腹泻的病原体对于采取措施预防和控制这种疾病至关重要。苏丹有关腹泻的报道也非常少。我们的研究旨在确定苏丹喀土穆儿童中特定原生动物病原体（组织溶解恩塔米巴虫、副隐孢子虫和贾第鞭毛虫）的流行率：我们对 2014 年 4 月至 12 月间因急性腹泻住院的五岁以下儿童进行了横断面调查。我们收集了腹泻粪便样本，并使用多重实时 PCR 检测了组织溶解酵母菌、副酵母菌和贾第鞭毛虫：本研究共纳入了 437 名急性腹泻患儿，其中年龄小于 2 岁的患儿发病率较高（403，92.2%）。155人（35.5%）感染了肠道寄生虫，16人（10.3%）合并感染。贾第虫（18.8%）和寄生虫（15.8%）是最常见的寄生虫，其次是组织溶解性大肠杆菌（0.9%）。寄生虫感染率最高的是 2 岁以下组（92.3%），最低的是 2-4 岁组（7.3%）。男孩的感染率（67.1%）高于女孩（32.9%）。雨季（8 月至 12 月）的原生动物感染率较高（92.2%），旱季（4 月至 6 月）的感染率较低（7.8%）。讨论本研究表明，在喀土穆地区的腹泻儿童中，贾第虫属和副猪痢疾杆菌的感染率很高，而且多重实时方法在揭示致病原生动物病原体方面非常有用。我们的研究结果凸显了制定干预措施和控制策略的必要性，以应对该地区的儿童寄生虫腹泻问题。

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来源期刊

F1000Research Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

5.00

自引率

0.00%

发文量

1646

审稿时长

1 weeks

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