Effects of Benzo[α]pyrene on Mucus Secretion and Tissue Remodeling in a Rat Model of Allergic Rhinitis.

IF 1.3 4区医学 Q3 OTORHINOLARYNGOLOGY Annals of Otology Rhinology and Laryngology Pub Date : 2024-08-15 DOI:10.1177/00034894241275449

Jian Liu, Guohui Chen, Xuemei Qin, Qing Sun, Zhiqiang Guo

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Abstract

Objectives: Exposure to benzo[α]pyrene (BaP) increases the incidence and severity of allergic rhinitis (AR), but the underlying mechanisms remain unclear. Thus, we investigated the in vivo effects of BaP exposure on mucus hypersecretion and tissue remodeling in a rat model of AR.

Methods: Female Sprague-Dawley rats were randomly divided into 4 groups: a negative control group, a group of healthy rats exposed to BaP, a group of rats with ovalbumin (OVA)-induced AR, and a group of AR model rats exposed to BaP. Nasal symptoms and levels of OVA-specific serum immunoglobulin E (IgE) were measured in each individual rat. Moreover, examination of goblet cell hyperplasia and collagen deposition was carried out with periodic acid-Schiff (PAS) staining and Masson trichrome (MT) staining. Mucin 5AC (MUC5AC) expression was assessed by immunohistochemistry.

Results: BaP significantly increased the number of sneezes, the number of nasal rubs and the levels of OVA-specific serum IgE in rats with AR. Statistically significant differences in goblet cell hyperplasia and collagen deposition were observed between the BaP-exposed AR model group and the AR model group. Immunohistochemical results showed that the nasal mucosa of AR model rats displayed markedly elevated MUC5AC expression after BaP exposure.

Conclusion: Our data indicate that mucus hypersecretion and the development of nasal remodeling might be pathophysiologic mechanisms underlying increased susceptibility to AR after exposure to BaP.

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苯并[α]芘对过敏性鼻炎大鼠模型粘液分泌和组织重塑的影响

目的：接触苯并[α]芘（BaP）会增加过敏性鼻炎（AR）的发病率和严重程度，但其潜在机制仍不清楚。因此，我们在大鼠 AR 模型中研究了暴露于 BaP 对粘液高分泌和组织重塑的体内影响：方法：将雌性 Sprague-Dawley 大鼠随机分为 4 组：阴性对照组、暴露于 BaP 的健康大鼠组、卵清蛋白（OVA）诱导的 AR 大鼠组和暴露于 BaP 的 AR 模型大鼠组。对每只大鼠的鼻部症状和卵清蛋白特异性血清免疫球蛋白 E (IgE) 水平进行了测量。此外，还使用周期性酸-希夫（PAS）染色法和马森三色染色法（MT）检查了鹅口疮细胞增生和胶原沉积情况。免疫组化法评估了粘蛋白 5AC（MUC5AC）的表达：结果：BaP 能明显增加 AR 大鼠的打喷嚏次数、擦鼻次数和 OVA 特异性血清 IgE 水平。BaP暴露的AR模型组与AR模型组之间在滤泡细胞增生和胶原沉积方面存在统计学意义上的明显差异。免疫组化结果显示，暴露于 BaP 后，AR 模型大鼠鼻粘膜的 MUC5AC 表达明显升高：我们的数据表明，粘液分泌过多和鼻腔重塑可能是暴露于 BaP 后增加 AR 易感性的病理生理机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Otology Rhinology and Laryngology 医学-耳鼻喉科学

CiteScore

3.10

自引率

7.10%

发文量

171

审稿时长

4-8 weeks

期刊介绍： The Annals of Otology, Rhinology & Laryngology publishes original manuscripts of clinical and research importance in otolaryngology–head and neck medicine and surgery, otology, neurotology, bronchoesophagology, laryngology, rhinology, head and neck oncology and surgery, plastic and reconstructive surgery, pediatric otolaryngology, audiology, and speech pathology. In-depth studies (supplements), papers of historical interest, and reviews of computer software and applications in otolaryngology are also published, as well as imaging, pathology, and clinicopathology studies, book reviews, and letters to the editor. AOR is the official journal of the American Broncho-Esophagological Association.