Improved and easy method for long-term in vitro culture of rumen ciliates Dasytricha ruminantium

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES MethodsX Pub Date : 2024-08-10 DOI:10.1016/j.mex.2024.102902
Qinhui Xu , Jinying He , Yujia Wang , Jie Xiong , Weiwei Qin , Jinmei Feng
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Abstract

The rumen ciliates are a diverse group of protozoa residing in the rumen of ruminant animals. They are primarily found in the orders Entodiniomorphida and Vestibuliferida, playing crucial roles in the digestion and breakdown of feed within the host's rumen, closely intertwined with the host's nutrient absorption. In vitro monocultures of representatives of rumen ciliates are important to better study them. So far, Entodinium caudatum and Epidinium caudatum, representatives of the order Entodiniomorphida, have been successfully cultivated as a monoculture in vitro. However, for the order Vestibuliferida, no representative species has been established a stable monoculture in vitro up to date, which hampers to study their physiology and metabolism. Therefore, we have developed a simple method for the in vitro cultivation of Dasytricha ruminantium, a representative rumen ciliate in the order Vestibuliferida. Utilizing an optimized culture medium with easily obtainable components, and the cultivation process is simple. This will facilitate further research in metabolism and other studies requiring large pure live materials.

  • 1.

    Filtration and separation for enriching D. ruminantium.

  • 2.

    A culture medium (DRM) suitable for the growth of D. ruminantium, with easily obtainable components.

  • 3.

    Simple cultivation process, facilitating the obtainment of a large number of monocultured D. ruminantium.

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瘤胃纤毛虫 Dasytricha ruminantium 长期体外培养的改进型简便方法
瘤胃纤毛虫是居住在反刍动物瘤胃中的一类多种多样的原生动物。它们主要分布在 Entodiniomorphida 和 Vestibuliferida 目中,在宿主瘤胃内消化和分解饲料的过程中发挥着至关重要的作用,与宿主的营养吸收密切相关。瘤胃纤毛虫代表的离体单培养对于更好地研究它们非常重要。迄今为止,Entodinium caudatum 和 Epidinium caudatum(Entodiniomorphida 目纤毛虫的代表)已经成功地进行了体外单一培养。然而,迄今为止,Vestibuliferida 目还没有一个代表物种在体外建立了稳定的单一栽培,这阻碍了对其生理和代谢的研究。因此,我们开发了一种体外培养瘤胃纤毛虫(Dasytricha ruminantium)的简单方法。该方法采用了易于获得的优化培养基,且培养过程简单。1.过滤和分离富集反刍动物。2.适合反刍动物生长的培养基(DRM),成分容易获得。3.培养过程简单,便于获得大量单培养的反刍动物。
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来源期刊
MethodsX
MethodsX Health Professions-Medical Laboratory Technology
CiteScore
3.60
自引率
5.30%
发文量
314
审稿时长
7 weeks
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