The protective effect of Cloprostenol on ischemia/reperfusion injury in rat ovary: Histopathologic and immunohistochemically evaluation: An experimental study

IF 1.6 4区医学 Q4 IMMUNOLOGY Transplant immunology Pub Date : 2024-08-14 DOI:10.1016/j.trim.2024.102108

Savas Karakus , Halef Okan Dogan , Mustafa Özkaraca

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Abstract

Objectıves

This study investigates whether Cloprostenol, a synthetic prostaglandin analog, could protect against ischemia/reperfusion (IR) injury in rat ovaries.

Methods

Adult female rats were divided into four groups: Sham groups, ischemia (IS) groups, ischemia/reperfusion (IR) groups, and Cloprostenol-treated (CT) groups. The IR injury model was established by clamping the ovarian pedicle for a specified period, followed by reperfusion. The CT group received a pre-treatment of Cloprostenol before inducing ischemia. Ovarian tissues were collected for histological, and immunohistochemical examination.

Results

The IS group exhibited severe morphological damage to ovarian tissues, including disrupted tissue architecture and increased apoptosis (p < 0.001). In contrast, the CT group displayed significantly improved ovarian histology, with notable preservation of ovarian tissue and reduced apoptotic activity (p < 0.01). Immunohistochemical analysis revealed that the levels of 8-Hydroxy-2-deoxyguanosine (8-OHdG), Caspase 3, Cyclooxygenase 2 (COX-2), and Interleukin 1 beta (IL-1β) staining, which were elevated in the IS and IR groups, were significantly diminished in the CT group (p < 0.05).

Conclusıon

Cloprostenol administration before IR injury in rat ovaries demonstrated a remarkable protective effect by improving histological damage and reducing DNA damage inflammation. These results highlight the therapeutic potential of Cloprostenol in safeguarding ovarian health against IR.

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氯前列烯醇对大鼠卵巢缺血再灌注损伤的保护作用：组织病理学和免疫组织化学评估：一项实验研究。

研究目的本研究探讨合成前列腺素类似物氯前列醇是否能保护大鼠卵巢免受缺血再灌注损伤。方法：成年雌性大鼠分为四组：缺血（IS）组、缺血/再灌注（IR）组和氯前列醇处理（CT）组。IR损伤模型是通过夹闭卵巢蒂一段时间，然后进行再灌注而建立的。CT 组在诱导缺血前接受氯前列醇预处理。收集卵巢组织进行组织学和免疫组化检查。结果：IS 组卵巢组织出现严重的形态学损伤，包括组织结构破坏和细胞凋亡增加（p

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来源期刊

Transplant immunology 医学-免疫学

CiteScore

2.10

自引率

13.30%

发文量

198

审稿时长

48 days

期刊介绍： Transplant Immunology will publish up-to-date information on all aspects of the broad field it encompasses. The journal will be directed at (basic) scientists, tissue typers, transplant physicians and surgeons, and research and data on all immunological aspects of organ-, tissue- and (haematopoietic) stem cell transplantation are of potential interest to the readers of Transplant Immunology. Original papers, Review articles and Hypotheses will be considered for publication and submitted manuscripts will be rapidly peer-reviewed and published. They will be judged on the basis of scientific merit, originality, timeliness and quality.