Accidental bufotoxin intoxication: Arenobufagin identification by liquid chromatography coupled to mass spectrometry

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY Practical Laboratory Medicine Pub Date : 2024-08-01 DOI:10.1016/j.plabm.2024.e00424

Alessandro Bonari , Mauro Leucio Mattei , Giovanni Cappelli , Francesca Romano , Nicoletta Cini , Francesca Luceri , Donato Squillaci , Stefano Dugheri , Alessandra Fanelli , Nicola Mucci

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Abstract

Background: Since ancient times, poisoning, even serious poisoning, has been known to occur during nature walks. Intentional or unintentional ingestion of toxins of animal origin is one of the possible causes of poisoning. Bufadienolide poisoning is a critical case. This is because of its high potency and its ability to cross the blood-brain barrier. Due to the rarity of these poisonings in humans in Central Europe, their identification is often difficult. The following is a case report of a poisoning by toad eggs in an Italina child, that presented vertigo, fussiness and sleepiness. A method of toxin identification using the prince of pharmacotoxicology, liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS), and an innovative reasoning were used. This method can be applied to other poisoning cases.

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意外布福毒素中毒：液相色谱-质谱法鉴定阿伦布法金

背景：自古以来，人们就知道在大自然漫步时会发生中毒事件，甚至是严重中毒。有意或无意摄入动物源毒素是中毒的可能原因之一。Bufadienolide 中毒是一个严重的案例。这是因为它的毒性很强，而且能够穿过血脑屏障。由于此类中毒在中欧地区非常罕见，因此通常很难鉴别。以下是一例伊塔利纳儿童蟾蜍卵中毒的病例报告，中毒者表现为眩晕、烦躁和嗜睡。该病例采用了药理毒理学、液相色谱法（LC）和串联质谱法（MS/MS）的毒素鉴定方法，并进行了创新性推理。该方法可应用于其他中毒病例。

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.