First report of detection of mcr-1 and virulence genes in avian pathogenic Escherichia coli in the center of Algeria.

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES Iranian journal of veterinary research Pub Date : 2024-01-01 DOI:10.22099/IJVR.2024.47413.6840
Z Halfaoui, H Rahab, R Achek, M N Menoueri
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Abstract

Background: Antimicrobial resistance in avian pathogenic Escherichia coli (APEC) represents a major concern in the avian industry worldwide and limited studies have investigated Colistin resistance among APEC in Algeria.

Aims: Investigate antibiotic resistance, in particular, Colistin, and mediated-Colistin resistance (mcr) genes, as well as the virulence genes in APEC.

Methods: One hundred E. coli were isolated from poultry suspected of colibacillosis. Antimicrobial susceptibility testing was done on 14 antibiotics by the disk diffusion method. Colistin minimum inhibitory concentration (MIC) was assessed by the broth microdilution method. Using multiplex PCR, mcr genes (mcr-1 to 5) and 7 virulence-related genes were investigated in Colistin-resistant isolates.

Results: Results showed high resistance to Tetracycline (99%), Nalidixic acid (92%), Doxycycline (90%), Ampicillin (89%), Ofloxacin (74%), Sulfamethoxazole-Trimethoprim (72%), and Amoxicillin-Clavulanic acid (57%); in addition, 92% of isolates were multidrug resistant. The rate of resistance to Colistin was 27% (27/100) of which 96.3% (26/27) of isolates carried the mcr-1 gene. Twenty-five of the Colistin-resistant isolates (92.59%) had at least three virulence genes. The most frequently isolated virulence genes were: fim H (96.3%) followed by hlyF, iroN, and iss (77.7%, each), iutA and ompT were found in 59.25% and 55.5% of isolates, respectively. The most prevalent combination of virulence factors was hlyF-iss-iroN-iutA-ompT-fimH.

Conclusion: This is the first report which highlighted Colistin resistance with the detection of mcr-1 in APEC isolates in the area of study. Colistin resistance and carriage of mcr-1 in virulent and multidrug-resistant isolates of E. coli are alarming and a surveillance program to limit the spread of these pathogens is mandatory.

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首次报告在阿尔及利亚中部禽类致病性大肠杆菌中检测到 mcr-1 和毒力基因。
背景:目的:调查禽致病性大肠埃希菌(APEC)的抗生素耐药性,特别是可乐定和介导的可乐定耐药基因(mcr),以及 APEC 的毒力基因:方法:从疑似大肠杆菌病的家禽中分离出 100 个大肠杆菌。采用磁盘扩散法对 14 种抗生素进行了抗菌药敏感性测试。肉汤微量稀释法评估了秋水仙素的最低抑菌浓度(MIC)。利用多重 PCR 技术,对耐药菌株的 mcr 基因(mcr-1 至 5)和 7 个毒力相关基因进行了研究:结果显示,对四环素(99%)、萘啶酸(92%)、强力霉素(90%)、氨苄西林(89%)、氧氟沙星(74%)、磺胺甲噁唑-三甲双胍(72%)和阿莫西林-克拉维酸(57%)的耐药性较高;此外,92%的分离株对多种药物耐药。对秋水仙素的耐药率为 27%(27/100),其中 96.3%(26/27)的分离株携带 mcr-1 基因。25个耐秋水仙素的分离株(92.59%)至少有三种毒力基因。最常分离到的毒力基因是:fim H(96.3%),其次是 hlyF、iroN 和 iss(各占 77.7%),在 59.25% 和 55.5% 的分离物中分别发现了 iutA 和 ompT。最常见的致病因子组合是 hlyF-iss-iroN-iutA-ompT-fimH :这是第一份在研究地区的 APEC 分离物中检测到 mcr-1 并突出显示对 Colistin 产生耐药性的报告。大肠杆菌的耐药和耐多药分离株的耐秋水仙素和携带 mcr-1 的情况令人担忧,因此必须实施监控计划以限制这些病原体的传播。
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来源期刊
CiteScore
1.70
自引率
8.30%
发文量
10
期刊介绍: The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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