Magesh Karuppur Thiagarajan, Deepak Chandrasekharan, Ramya Mahalingam, Aravindhan Ravi
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引用次数: 0
Abstract
Background: For decades, Wrightia tinctoria (W. tinctoria) has been important in the field of traditional medicine. The identification and isolation of numerous chemically potent and pharmaceutically advantageous phytochemicals isolated from this plant extract has contributed to its resurrection into evidence-based modern medicine. This plant is inexpensive and indigenous to the Indian subcontinent.
Materials and method: An in vitro study was carried out using KB (KERATIN-forming tumour cell line HeLa) oral cancer cell line. The control drug doxorubicin along with the test drug W. tinctoria was tested for anti-cancer potential by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay and for apoptotic activity by using DNA fragmentation assay and western blotting assay.
Results: The study was conducted using MTT assay to determine the cytotoxicity of plant extract against oral cancer cell line by determining the IC50 values at the end of 24 hours by the MTT calorimetric assay. The IC50 value of 48.89 and 9.62 was arrived for the W. tinctoria extract and doxorubicin, respectively, by using MTT calorimetric assay. DNA fragmentation assay showed a ladder pattern when treated with 59 μg/mL extract of W. tinctoria, and western blot analysis revealed the presence of cleaved caspase 3, confirming the apoptotic potential of the extract.
Conclusion: The current study demonstrated that the plant extract has effective anti-cancer and apoptotic potential and can be used as a natural source for the production of new anti-cancer and cytotoxic agents against oral cancer.
期刊介绍：
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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