Circular RNA LDLRAD3 promotes gastric cancer progression by upregulating COL4A5 through sponging miR-h37.

Chenghui Li, Xiao Xing, Sinian Huang, Ting Zhu, Bin Yan
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Abstract

Background: Circular RNAs play an important role in the development of gastric cancer (GC). circ-low-density lipoprotein receptor class A domain containing 3 (LDLRAD3) has been confirmed to be related to GC progression. miR-137 is also a suppressor in GC. However, the impact of the interaction between circ-LDLRAD3 and miR-137 on the progression of GC remains unclear at present.

Methods: The study identified expression level differences of circ-LDLRAD3, miR-137, and COL4A5 in GC pathological specimens compared to normal tissue samples. Furthermore, through in vitro experiments, including flow cytometry, cell counting kit-8 (CCK-8) assays, wound healing, Western blotting, and colony formation assays, we further explored the molecular regulatory mechanisms by which these factors promote the progression of GC.

Results: In this study, circ-LDLRAD3 was confirmed to have higher expression, and miR-137 had lower expression in GC tissues and cell lines. circ-LDLRAD3 knockdown and miR-137 overexpression promoted apoptosis and inhibited proliferation, migration, and invasion in GC cell lines. Further experiments validated that COL4A5 had a positive relationship with GC and that circ-LDLRAD3 promoted the expression of COL4A5. circ-LDLRAD3 could be sponged and inhibited by miR-137 in GC cells. As a result, the promotional effect of circ-LDLRAD3 on COL4A5 was counteracted by miR-137.

Conclusion: Our study showed that the knockdown of circ-LDLRAD3 suppressed the development of GC by regulating the miR-137/COL4A5 axis.

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环状 RNA LDLRAD3 通过海绵状 miR-137 上调 COL4A5 从而促进胃癌的进展。
背景:已证实circ-LDLRAD3与胃癌的进展有关,而miR-137也是胃癌的抑制因子。然而,目前尚不清楚 circ-LDLRAD3 和 miR-137 之间的相互作用对胃癌进展的影响:研究发现,与正常组织样本相比,胃癌病理样本中 circ-LDLRAD3、miR-137 和 COL4A5 的表达水平存在差异。此外,通过流式细胞术、CCK-8 检测、伤口愈合、Western 印迹和集落形成检测等体外实验,我们进一步探讨了这些因子促进胃癌进展的分子调控机制:结果:本研究证实,在胃癌组织和细胞系中,circ-LDLRAD3的表达量较高,而miR-137的表达量较低;circ-LDLRAD3敲除和miR-137过表达可促进胃癌细胞系的凋亡,抑制其增殖、迁移和侵袭。进一步的实验验证了 COL4A5 与 GC 存在正相关关系,而 circ-LDLRAD3 可促进 COL4A5 的表达。因此,circ-LDLRAD3对COL4A5的促进作用被miR-137抵消:总之,我们发现通过调节 miR-137/COL4A5 轴,敲除 circ-LDLRAD3 可抑制 GC 的发展。
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