Polymers produced with depolymerase

IF 12.9 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Nature chemical biology Pub Date : 2024-08-20 DOI:10.1038/s41589-024-01723-0
Russell Johnson
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用解聚酶生产的聚合物
许多塑料在环境中无法降解。改用可生物降解和可堆肥的聚合物进行生产,为设计塑料固有的有限使用寿命提供了一条途径,从而确保废塑料不会在环境中持续存在。聚乳酸(PLA)是一种广泛使用的生物聚合物,但只有在工业堆肥的高温条件下才能有效降解;在家庭堆肥和土壤条件下的生物降解率很低。首先,Guicherd 等人从嗜热细菌 Actinomadura keratinilytica T16-1 中分离并纯化出一种新的聚乳酸解聚酶,他们称之为 PAM。表征结果表明,与蛋白酶 K(一种常用酶)相比,PAM 对聚乳酸解聚更有活性。随后,研究小组利用 X 射线晶体学技术解决了 PAM 的结构问题,从而建立了其解聚酶活性模型。接下来,研究小组在 PAM 中引入突变,创造出一种高效的解聚酶。聚乳酸塑料的加工过程在高温下进行,这会使酶失活,因此 Guicherd 等人通过将 PAM 的有益突变接枝到恒温支架上,创造出了一种超恒温聚乳酸水解酶。
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来源期刊
Nature chemical biology
Nature chemical biology 生物-生化与分子生物学
CiteScore
23.90
自引率
1.40%
发文量
238
审稿时长
12 months
期刊介绍: Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.
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