Insights into the global genomic features of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2024-09-02 DOI:10.1093/jambio/lxae217

Isabela C Campos, Felipe Pinheiro Vilela, Mauro de M S Saraiva, Angelo Berchieri Junior, Juliana Pfrimer Falcão

{"title":"Insights into the global genomic features of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum.","authors":"Isabela C Campos, Felipe Pinheiro Vilela, Mauro de M S Saraiva, Angelo Berchieri Junior, Juliana Pfrimer Falcão","doi":"10.1093/jambio/lxae217","DOIUrl":null,"url":null,"abstract":"Aims: Characterize global genomic features of 86 genomes of Salmonella Gallinarum (SG) and Pullorum (SP), which are important pathogens causing systemic infections in poultry.Methods and results: All genomes harbored efflux pump encoding gene mdsA and gold tolerance genes golS and golT. Aminoglycoside (aac(6')-Ib, aadA5, aph(6)-Id, aph(3'')-Ib, ant(2'')-Ia), beta-lactam (blaTEM-1, blaTEM-135), efflux pump (mdsB), fosfomycin (fosA3), sulfonamide (sul1, sul2), tetracycline [tet(A)], trimethoprim (dfrA17), acid (asr), and disinfectant (qacEdelta1) resistance genes, gyrA, gyrB, and parC quinolone resistance point mutations, and mercury tolerance genes (mer) were found in different frequencies. Additionally, 310 virulence genes, pathogenicity islands (including SPI-1, 2, 3, 4, 5, 6, 9, 10, 12, 13, and 14), plasmids [IncFII(S), ColpVC, IncX1, IncN, IncX2, and IncC], and prophages (Fels-2, ST104, 500465-1, pro483, Gifsy-2, 103 203_sal5, Fels-1, RE-2010, vB_SenS-Ent2, and L-413C) were detected. MLST showed biovar-specific sequence types, and core genome MLST showed country-specific and global-related clusters.Conclusion: SG and SP global strains carry many virulence factors and important antimicrobial resistance genes. The diverse plasmids and prophages suggest genetic variability. MLST and cgMLST differentiated biovars and showed profiles occurring locally or worldwide.","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxae217","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aims: Characterize global genomic features of 86 genomes of Salmonella Gallinarum (SG) and Pullorum (SP), which are important pathogens causing systemic infections in poultry.

Methods and results: All genomes harbored efflux pump encoding gene mdsA and gold tolerance genes golS and golT. Aminoglycoside (aac(6')-Ib, aadA5, aph(6)-Id, aph(3'')-Ib, ant(2'')-Ia), beta-lactam (blaTEM-1, blaTEM-135), efflux pump (mdsB), fosfomycin (fosA3), sulfonamide (sul1, sul2), tetracycline [tet(A)], trimethoprim (dfrA17), acid (asr), and disinfectant (qacEdelta1) resistance genes, gyrA, gyrB, and parC quinolone resistance point mutations, and mercury tolerance genes (mer) were found in different frequencies. Additionally, 310 virulence genes, pathogenicity islands (including SPI-1, 2, 3, 4, 5, 6, 9, 10, 12, 13, and 14), plasmids [IncFII(S), ColpVC, IncX1, IncN, IncX2, and IncC], and prophages (Fels-2, ST104, 500465-1, pro483, Gifsy-2, 103 203_sal5, Fels-1, RE-2010, vB_SenS-Ent2, and L-413C) were detected. MLST showed biovar-specific sequence types, and core genome MLST showed country-specific and global-related clusters.

Conclusion: SG and SP global strains carry many virulence factors and important antimicrobial resistance genes. The diverse plasmids and prophages suggest genetic variability. MLST and cgMLST differentiated biovars and showed profiles occurring locally or worldwide.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

洞察肠炎沙门氏菌 Gallinarum 和 Pullorum 的全球基因组特征。

目的：描述引起家禽全身感染的重要病原体加里纳氏沙门氏菌（SG）和普拉伦氏沙门氏菌（SP）的 86 个基因组的全局基因组特征。方法与结果：所有基因组都含有外排泵编码基因 mdsA 以及耐金基因 golS 和 golT。氨基糖苷类（aac(6')-Ib、aadA5、aph(6)-Id、aph(3'')-Ib、ant(2'')-Ia）、β-内酰胺类（blaTEM-1、blaTEM-135）、外排泵（mdsB）、磷霉素（fosA3）、磺胺类（sul1、sul2）、四环素类（tet(A)）、耐酸（asr）和耐消毒剂（qacEdelta1）基因，gyrA、gyrB 和 parC 喹诺酮耐药点突变，以及汞耐受基因（mer）都以不同的频率被发现。此外，还检测到 310 个毒力基因、致病岛（包括 SPI-1、2、3、4、5、6、9、10、12、13、14）、质粒（IncFII(S)、ColpVC、IncX1、IncN、IncX2、IncC）和噬菌体（Fels-2、ST104、500465-1、pro483、Gifsy-2、103203_sal5、Fels-1、RE-2010、vB_SenS-Ent2、L-413C）。MLST 显示了生物菌株特异性序列类型，核心基因组 MLST 显示了国家特异性和全球相关集群：结论：SG 和 SP 全球菌株携带多种毒力因子和重要的抗菌药耐药性基因。结论：SG 和 SP 全球菌株携带多种致病因子和重要的抗菌基因。MLST 和 cgMLST 对生物种进行了区分，并显示了发生在本地或全球的特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.