Preparation for targeted sterile insect technique to control invasive Aedes aegypti (Diptera: Culicidae) in southern California: dose-dependent response, survivorship, and competitiveness.

Abstract

Aedes aegypti is fast spreading across California, with over 300 cities within 22 central and southern counties being infested since its introduction in 2013. Due to its cryptic breeding habitats, control efforts have not been successful so far. This calls for innovative tools such as sterile insect technique (SIT) to reinforce the existing integrated pest management (IPM). Here, we assessed fitness, survivorship, and dose response of X-ray irradiated male Ae. aegypti in California. Locally acquired Ae. aegypti eggs were hatched and reared in temperature-controlled laboratory setting at the West Valley Mosquito and Vector Control District in Ontario, California. Freshly emerged adult male mosquitoes were manually separated using motor-operated aspirators and treated with X-ray radiation at different dosage (42-60 Gy). Dose response of irradiated males was analyzed and induced sterility determined. Survivorship of males treated with different X-ray doses was compared. Fecundity of females that mated with irradiated males at different X-ray doses was generally comparable. Overall, induced sterility increased with higher X-ray doses. Nulliparous females that mated with male Ae. aegypti treated with 55-60 Gy laid eggs with over 99% sterility. Non-irradiated male mosquitoes had higher survivorship (mean = 0.78; P = 0.0331) than irradiated mosquitoes (mean range = 0.50-0.65). The competitiveness index of irradiated males decreased with increasing X-ray treatment doses, 1.14 at 55 Gy and 0.49 at 60 Gy, and this difference was significant (P < 0.01). Irradiated males showed high survivorship and competitiveness-key for the anticipated SIT application for the control of invasive Ae. aegypti in California.