Role of sodium pyrophosphate and catechin in the enzymatic hydrolysis of oxidatively damaged myofibrillar protein gels in vitro: Mechanistic insights

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED Food Chemistry Pub Date : 2024-08-14 DOI:10.1016/j.foodchem.2024.140884

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Abstract

This study investigated the effects of sodium pyrophosphate (SPP) and catechin (C) on the in vitro enzymatic digestion of oxidatively damaged myofibrillar protein (MP) gel. The results indicated that SPP increased the β-sheet content and the gastric digestibility of the MP gel, while C hindered the transition from α-helix to β-sheet structure, leading to decreased digestibility. Notably, neither compound significantly affected intestinal digestibility. Furthermore, SPP and C significantly enhanced the antioxidant activity of MP gel digestion products. Notably, their synergistic hydrolysis products, simulating both gastric and gastrointestinal stages, chelated 91.4 % and 89.1 % of Fe²⁺ and scavenged 59.4 % and 77.6 % of hydroxyl radicals, respectively. Moreover, the final digestion products of the MP gel treated with SPP and C exhibited the highest content of negatively charged amino acids and absolute Zeta potential values. Overall, this study demonstrated that incorporating SPP and C could positively impact the digestion of oxidatively damaged MP gels.

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焦磷酸钠和儿茶素在体外酶水解氧化损伤的肌纤维蛋白凝胶中的作用：机理启示

本研究探讨了焦磷酸钠（SPP）和儿茶素（C）对氧化损伤肌纤蛋白（MP）凝胶体外酶解的影响。结果表明，SPP 增加了 MP 凝胶的 β 片层含量和胃消化率，而 C 则阻碍了 α 螺旋结构向 β 片层结构的转变，导致消化率下降。值得注意的是，这两种化合物都不会明显影响肠道消化率。此外，SPP 和 C 还能明显提高 MP 凝胶消化产物的抗氧化活性。值得注意的是，它们的协同水解产物在模拟胃和肠胃阶段时，分别螯合了 91.4% 和 89.1% 的 Fe2+，清除了 59.4% 和 77.6% 的羟基自由基。此外，经 SPP 和 C 处理的 MP 凝胶的最终消化产物显示出最高的带负电氨基酸含量和绝对 Zeta 电位值。总之，这项研究表明，加入 SPP 和 C 可对氧化损伤的 MP 凝胶的消化产生积极影响。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.