Dissolved oxygen fluctuation alters dissolved organic carbon stability and can accumulate resistant fraction

Abstract

Recurrent dissolved oxygen (DO) depletion is common in many estuaries and coastal areas worldwide. However, its impact on the accumulation of resistant dissolved organic carbon (DOC) remains controversial. To address how recurrent DO depletion affects the degradation and stability of marine DOC, a series of DOC degradation incubations in a particulate-dissolved coexisting system in April 2021 were conducted, followed by a field investigation in the East China Sea (ECS) in October. In the ECS, we found that DO ranged from 4.71 to 7.40 mg/L in the near-bottom waters, while the degradation index (DI), an indicator of amino acids molecules, increased from −0.78–2.84, suggesting an accumulation of labile DOC over DO depletion. This is further supported by the bacteria community composition that showed an increase in anaerobic metabolism families when DO <6.29 mg/L. Laboratory incubation revealed strong labile DOC release (∼ 243 ± 38 μmol/L) from particulate organic carbon (POC) over oxygen depletion, followed by significant DOC loss in either anoxic-oxic or oxic-oxic groups. The degraded original DOC was slightly less in the oxygen depletion-treated group compared to the oxic group, and the resistant proportion was estimated as 1% ∼ 8% after 1–14 days of anoxic treatments. The resistant DOC accumulation after DO alleviation for 30 days was much less in amount when compared to the big release of labile DOC over DO depletion (48 vs. 243 μmol/L) and we believed that the lack of observed resistant DOC accumulation in the field observation was due to the overlap of multiple anoxic-oxic cycling processes and the masking effect of newly labile DOC released from the POC.