Effect of abemaciclib and curcumin administration on sex hormones, reproductive functions, and oxidative DNA expression in rats.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-08-21 DOI:10.1080/10520295.2024.2389524

Zübeyir Huyut, Bünyamin Uçar, Kenan Yıldızhan, Fikret Altındağ, Mehmet Tahir Huyut

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Abstract

This study investigated whether abemaciclib (ABE) administration had any adverse effects on ovarian and sex hormones in female rats, and the protective effect of curcumin. Forty female rats were equally divided into the sham control, DMSO, curcumin (CMN), ABE, and ABE+CMN groups. Pharmaceuticals were administered by gavage daily for 28 days. Serum sex hormones were measured in an autoanalyzer operating with a microparticle immunoassay method. In addition, histopathological examination and 8-OHdG expression were performed on the ovarian tissue. Progesterone and testosterone levels were significantly decreased, while estradiol levels were significantly increased, in the ABE group compared to the sham and DMSO groups. In addition, there were significant differences in sex hormone levels in the CMN and/or CMN+ABE groups compared to the ABE group. There was decreased expression of 8-OHdG in the ABE+CMN group compared to the ABE or CMN only groups. This study exhibited that ABE administration can adversely affect functions and histology of the ovarian tissue, but CMN therapy may be protective against the adverse effects on ovarian in ABE-induced rats.

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服用阿柏西尼和姜黄素对大鼠性激素、生殖功能和氧化 DNA 表达的影响

本研究探讨了服用阿贝替尼（ABE）是否会对雌性大鼠的卵巢和性激素产生不良影响，以及姜黄素的保护作用。40 只雌性大鼠被平均分为假对照组、DMSO 组、姜黄素（CMN）组、ABE 组和 ABE+CMN 组。每天灌胃给药，连续 28 天。血清性激素用微粒子免疫分析法的自动分析仪进行测定。此外，还对卵巢组织进行了组织病理学检查和 8-OHdG 表达。与假体组和 DMSO 组相比，ABE 组的孕酮和睾酮水平明显下降，而雌二醇水平则明显上升。此外，CMN组和/或CMN+ABE组的性激素水平与ABE组相比也有明显差异。与 ABE 组或仅 CMN 组相比，ABE+CMN 组的 8-OHdG 表达量有所下降。这项研究表明，ABE 会对卵巢组织的功能和组织学产生不利影响，但 CMN 治疗可防止 ABE 诱导的大鼠卵巢受到不利影响。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.