A sex-specific homologue of waprin is essential for embryonic development in the red flour beetle, Tribolium castaneum.

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-08-21 DOI:10.1111/imb.12956

Chhavi Choudhary, Divyanshu Kishore, Keshav Kumar Meghwanshi, Vivek Verma, Jayendra Nath Shukla

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Abstract

Waprin, a WAP (Whey acidic protein) domain-containing extracellular secretory protein, is widely known for its antibacterial properties. In this study, a waprin homologue (Tc_wap^F) expressing in a female-specific manner was identified in Tribolium castaneum, through the analysis of sex-specific transcriptomes. Developmental- and tissue-specific profiling revealed the widespread expression of Tc_wap^F in adult female tissues, particularly in the ovary, gut and fatbody. This female-specific expression of Tc_wap^F is not regulated by the classical sex-determination cascade of T. castaneum, as we fail to get any attenuation in Tc_wap^F transcript levels in Tcdsx and Tctra (key players of sex determination cascade of T. castaneum) knockdown females. RNA interference-mediated knockdown of Tc_wap^F in females led to the non-hatching of eggs laid by these females, suggesting the crucial role of Tc_wap^F in the embryonic development in T. castaneum. This is the first report on the identification of a sex-specific waprin homologue in an insect and its involvement in embryonic development. Future investigations on the functional conservation of insect waprins and their mechanistic role in embryonic development can be exploited for improving pest management strategies.

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在红面粉甲虫（Tribolium castaneum）的胚胎发育过程中，waprin的性别特异性同源物是必不可少的。

Waprin是一种含WAP（乳清酸性蛋白）结构域的细胞外分泌蛋白，因其抗菌特性而广为人知。在这项研究中，通过分析性别特异性转录组，发现了一种以雌性特异性方式表达的Waprin同源物（Tc_wapF）。发育和组织特异性分析表明，Tc_wapF 在成年雌性组织中广泛表达，尤其是在卵巢、肠道和脂肪体中。Tc_wapF的这种雌性特异性表达不受T.castaneum经典性别决定级联的调控，因为在Tcdsx和Tctra（T.castaneum性别决定级联的关键角色）敲除的雌性中，Tc_wapF转录水平没有任何下降。RNA 干扰介导的雌性 Tc_wapF 基因敲除导致这些雌性所产的卵无法孵化，这表明 Tc_wapF 在蓖麻蝇胚胎发育过程中起着关键作用。这是首次报道在昆虫中鉴定性别特异性 waprin 同源物及其参与胚胎发育的情况。未来对昆虫 waprins 的功能保护及其在胚胎发育中的作用机制的研究可用于改进害虫管理策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).