Lectins CGL and MTL, representatives of mytilectin family, exhibit different antiproliferative activity in Burkitt's lymphoma cells.

IF 3.7 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY IUBMB Life Pub Date : 2024-08-21 DOI:10.1002/iub.2909
Alexandra S Kuzmich, Alina P Filshtein, Galina N Likhatskaya, Tatiana Y Gorpenchenko, Irina V Chikalovets, Tatyana O Mizgina, Kuo-Feng Hua, Gunhild von Amsberg, Sergey A Dyshlovoy, Oleg V Chernikov
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Abstract

Lectins are carbohydrate-binding proteins, whose biological effects are exerted via binding to glycoconjugates expressed on the surface of cells. Exposure to lectins can lead not only to a change in the structure and properties of cells but also to their death. Here, we studied the biological activity of lectins from the mussels Crenomytilus graynus (CGL) and Mytilus trossulus (MTL) and showed that these proteins can affect the proliferation of human lymphoma cells. Both lectins suppressed the formation of colonies as well as cell cycle progression. The mechanism of action of these lectins was not mediated by reactive oxygen species but included damaging of mitochondria, inhibition of key cell cycle points, and activation of MAPK signaling pathway in tumor cells. Computer modeling suggested that various effects of CGL and MTL on lymphoma cells may be due to the difference in the energy of binding of these lectins to carbohydrate ligands on the cell surface. Thus, molecular recognition of residues of terminal carbohydrates on the surface of tumor cells is a key factor in the manifestation of the biological action of lectins.

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凝集素 CGL 和 MTL 是蕈样凝集素家族的代表,它们在伯基特淋巴瘤细胞中表现出不同的抗增殖活性。
凝集素是一种碳水化合物结合蛋白,其生物效应是通过与细胞表面表达的糖凝集物结合而产生的。接触凝集素不仅会导致细胞结构和性质发生变化,还会导致细胞死亡。在这里,我们研究了来自贻贝 Crenomytilus graynus(CGL)和 Mytilus trossulus(MTL)的凝集素的生物活性,结果表明这些蛋白质能影响人类淋巴瘤细胞的增殖。这两种凝集素都能抑制菌落的形成和细胞周期的进展。这些凝集素的作用机制并非由活性氧介导,而是包括破坏线粒体、抑制细胞周期的关键点以及激活肿瘤细胞的 MAPK 信号通路。计算机建模表明,CGL 和 MTL 对淋巴瘤细胞的不同作用可能是由于这些凝集素与细胞表面碳水化合物配体结合的能量不同造成的。因此,对肿瘤细胞表面末端碳水化合物残基的分子识别是凝集素发挥生物作用的关键因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
IUBMB Life
IUBMB Life 生物-生化与分子生物学
CiteScore
10.60
自引率
0.00%
发文量
109
审稿时长
4-8 weeks
期刊介绍: IUBMB Life is the flagship journal of the International Union of Biochemistry and Molecular Biology and is devoted to the rapid publication of the most novel and significant original research articles, reviews, and hypotheses in the broadly defined fields of biochemistry, molecular biology, cell biology, and molecular medicine.
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