Exploring the Nexus of Steroidal Hormone Receptor, Uterine VEGF Expression and NADPH-d Interaction in Buffalo Uterus During Oestrous Cycle With Seasonal Variation.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Reproduction in Domestic Animals Pub Date : 2024-08-01 DOI:10.1111/rda.14710

Sagarika Dehury, Varinder Uppal, Devendra Pathak, Anuradha Gupta

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Abstract

The reproductive efficiency in buffalo is highly influenced by seasonal variability. Angiogenesis in the reproductive cycle is important for optimal physiological functioning of uterus. Estrogen receptor-α (ERα), vascular endothelial growth factor (VEGF) and reduced nicotinamide adenine dinucleotide phosphatase diaphorase (NADPH-d) are vital indicators for the uterine angiogenic process. This study was conducted to see the effect of season on the expression of different uterine angiogenic factors. Season wise (winter and summer) and phase wise (follicular and luteal), immune staining intensity of buffalo uterus was measured by calculating the optical density value (OD) for ERα and VEGF. Percentage of immuno-positive cell count for ERα was done. Histoenzymic NADPH-d expression was analysed. Expression of all these factors increased during follicular phase of oestrous cycle in order to support the angiogenesis; however, the expression was significantly lower (p ≤ 0.05) in term of OD value as well as percentage count of immuno-positive cells during summer season indicating lower angiogenic activity that subsequently affected reproduction in buffalo.

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探索水牛子宫发情周期中类固醇激素受体、子宫血管内皮生长因子表达与 NADPH-d 相互作用的联系与季节变化

水牛的繁殖效率受季节变化的影响很大。生殖周期中的血管生成对子宫的最佳生理功能非常重要。雌激素受体-α（ERα）、血管内皮生长因子（VEGF）和还原型烟酰胺腺嘌呤二核苷酸磷酸酶（NADPH-d）是子宫血管生成过程的重要指标。本研究旨在了解季节对不同子宫血管生成因子表达的影响。从季节（冬季和夏季）和阶段（卵泡期和黄体期）来看，通过计算ERα和VEGF的光密度值（OD）来测量水牛子宫的免疫染色强度。计算ERα免疫阳性细胞的百分比。分析了组织酶NADPH-d的表达。在发情周期的卵泡期，所有这些因子的表达量都有所增加，以支持血管生成；但在夏季，这些因子的表达量（OD 值和免疫阳性细胞的百分比）显著降低（p ≤ 0.05），表明血管生成活性降低，进而影响了水牛的繁殖。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.