Quantitative proteomic analysis of PK-15 cells infected with porcine circovirus type 3 using 4D-DIA approach.

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-08-22 DOI:10.1007/s11259-024-10501-1
Dan Wang, Jiangwei Song, Jing Wang, Rong Quan
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Abstract

Porcine circovirus type 3 (PCV3) infection is clinically related to various diseases, including porcine dermatitis and nephrotic syndrome (PDNS)-like disease, respiratory disease, reproductive disorders, and gastrointestinal and neurological diseases. Since PCV3 infection was discovered in 2016, it has developed rapidly and has attracted much attention worldwide. However, specific preventive and therapeutic interventions are currently lacking. In this study, four-dimensional (4D) data-independent acquisition (DIA)-based quantitative proteomics detection combined with bioinformatics analysis were employed to quantitatively identify the differentially expressed proteins in PK-15 cells from the PCV3-infected group compared with those from the uninfected control group. A total of 194 cellular proteins were significantly altered in response to PCV3 infection, including 58 upregulated proteins and 136 downregulated proteins. In our Gene Ontology (GO) enrichment analysis, these differentially expressed proteins were mostly associated with cellular anatomical entities, binding, cellular processes, biological regulation, catalytic activity, metabolic processes, developmental processes, protein-containing complexes and responses to stimuli. Our Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were predominantly involved in metabolic pathways, the cAMP signaling pathway, protein processing in the endoplasmic reticulum, the PI3K-Akt signaling pathway, and the calcium signaling pathway. For the experiments, Western blotting (WB) was used to confirm the changes in important molecules. The differentially expressed proteins identified should contribute to a greater understanding of the mechanism of PCV3 replication and pathogenesis, as well as the host response.

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利用 4D-DIA 方法对感染猪圆环病毒 3 型的 PK-15 细胞进行定量蛋白质组分析。
猪圆环病毒3型(PCV3)感染在临床上与多种疾病有关,包括猪皮炎肾病综合征(PDNS)样病、呼吸道疾病、繁殖障碍、胃肠道和神经系统疾病等。自 2016 年发现 PCV3 感染以来,该病发展迅速,引起了全世界的广泛关注。然而,目前尚缺乏具体的预防和治疗干预措施。本研究采用基于四维(4D)数据独立采集(DIA)的定量蛋白质组学检测,结合生物信息学分析,定量鉴定了PCV3感染组与未感染对照组PK-15细胞中差异表达的蛋白质。共有194个细胞蛋白在PCV3感染后发生了显著变化,包括58个上调蛋白和136个下调蛋白。在我们的基因本体(GO)富集分析中,这些差异表达的蛋白质大多与细胞解剖实体、结合、细胞过程、生物调控、催化活性、代谢过程、发育过程、含蛋白复合物和对刺激的反应有关。京都基因与基因组百科全书》(KEGG)分析显示,DEPs主要参与代谢途径、cAMP信号途径、内质网蛋白质加工、PI3K-Akt信号途径和钙信号途径。实验中使用了 Western 印迹(WB)来确认重要分子的变化。所发现的表达不同的蛋白质有助于进一步了解 PCV3 复制和致病机制以及宿主反应。
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来源期刊
Veterinary Research Communications
Veterinary Research Communications 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
173
审稿时长
3 months
期刊介绍: Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
期刊最新文献
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