Reduce energy consumption in your laboratory - switch ultra-low temperature freezers from - 80 °C to -70 °C. A pilot study on short term storage of plasma samples for coagulation testing.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-10-01 Epub Date: 2024-08-24 DOI:10.1080/00365513.2024.2394981
Sumangala Bhattacharya, Peter H Nissen
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Abstract

It is common practice in laboratories to store biological samples in ultra-low temperature (ULT) freezers. There is growing interest in raising the temperature of ULT freezers in order to save energy and reduce expenses, as energy conservation becomes increasingly important and sustainable laboratory practices gain popularity. In our laboratory, plasma samples are stored for three months for diagnostic purposes. We therefore took the opportunity to investigate the effect of two different storage temperatures (-70 °C vs -80 °C), on activated partial thromboplastin time (APTT), factor VIII (FVIII), international normalized ratio (INR) and factor VII (FVII) measurements on paired plasma samples collected from 26 individuals after three months of storage. Automated coagulation analysers CS-5100 and ACL TOP were used to perform the tests. We found no consistent difference between the two storage temperatures for any of the four coagulation parameters (all p-values > 0.05). We conclude that the temperature of ULT freezers used to store plasma samples for APTT, FVIII, INR, and FVII measurements can be safely increased from -80 to -70 °C without affecting the stability of the samples.

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降低实验室能耗--将超低温冷冻机的温度从-80 °C降至-70 °C。凝血测试血浆样本短期储存试验研究。
将生物样本储存在超低温(ULT)冷冻箱中是实验室的常见做法。随着节能越来越重要,以及可持续实验室实践的普及,人们对提高超低温冷冻箱的温度以节约能源和减少开支越来越感兴趣。在我们的实验室中,血浆样本需要储存三个月用于诊断。因此,我们借此机会研究了两种不同储存温度(-70 °C 与 -80 °C)对活化部分凝血活酶时间 (APTT)、因子 VIII (FVIII)、国际标准化比值 (INR) 和因子 VII (FVII) 测量的影响。测试使用 CS-5100 和 ACL TOP 自动凝血分析仪进行。我们发现两种储存温度对四项凝血参数中的任何一项都没有一致的差异(所有 p 值均大于 0.05)。我们的结论是,用于储存 APTT、FVIII、INR 和 FVII 测量血浆样本的超低温冷冻箱的温度可以从 -80 ℃ 安全地升高到 -70 ℃,而不会影响样本的稳定性。
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来源期刊
CiteScore
3.50
自引率
4.80%
发文量
85
审稿时长
4-8 weeks
期刊介绍: The Scandinavian Journal of Clinical and Laboratory Investigation is an international scientific journal covering clinically oriented biochemical and physiological research. Since the launch of the journal in 1949, it has been a forum for international laboratory medicine, closely related to, and edited by, The Scandinavian Society for Clinical Chemistry. The journal contains peer-reviewed articles, editorials, invited reviews, and short technical notes, as well as several supplements each year. Supplements consist of monographs, and symposium and congress reports covering subjects within clinical chemistry and clinical physiology.
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