Assessment of cytoprotective and genoprotective effects of Moringa oleifera and Tinospora cordifolia extracts in vitro.

IF 2.2 4区医学 Q3 TOXICOLOGY Toxicology Research Pub Date : 2024-08-22 eCollection Date: 2024-08-01 DOI:10.1093/toxres/tfae133

Preeti Bagri, Vinod Kumar, Kanisht Batra

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Abstract

Background: Moringa oleifera and Tinospora cordifolia is extensively used as an ingredient of food and in traditional medicine for the management of a variety of diseases.

Material and methods: The extracts of leaf of Moringa oleifera and stem of Tinospora cordifolia were assessed to examine their ability to inhibit the oxidative DNA damage (by DNA protection assay), cytoprotective and genoprotective potential (by Comet assay) in V79 cells individually and in combinations.

Result: It was found that these extracts could significantly inhibit the OH-dependent damage of pUC18 plasmid DNA. M. oleifera extract (160 and 320 μg/mL) and Tinospora cordifolia extract (640, 1,280 and 2,560 μg/mL) individually showed higher DNA protection activity. M. oleifera (1,280 μg/mL) combined with Tinospora cordifolia (640 μg/mL) showed best cytoprotective and genoprotective activities among different concentration combinations and various concentrations of individual plants in V79 cell line against hydrogen peroxide induced cytotoxicity and genotoxicity.

Conclusion: This study demonstrates the cytoprotective and genoprotective activity of M. oleifera and Tinospora cordifolia individually or in combination.

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在体外评估辣木和虎杖提取物的细胞保护和基因保护作用。

背景：Moringa oleifera 和 Tinospora cordifolia 被广泛用作食品原料和传统药物，用于治疗多种疾病：材料和方法：评估了油辣木叶和虎耳草茎的提取物，以检查它们单独和组合抑制 V79 细胞氧化 DNA 损伤（DNA 保护试验）、细胞保护和基因保护潜力（彗星试验）的能力：结果：研究发现，这些提取物能显著抑制 pUC18 质粒 DNA 的 OH 依赖性损伤。油橄榄提取物（160 和 320 μg/mL）和椴树提取物（640、1,280 和 2,560 μg/mL）单独显示出较高的 DNA 保护活性。在不同浓度组合和不同浓度的单株植物中，M. oleifera（1,280 μg/mL）与Tinospora cordifolia（640 μg/mL）组合在V79细胞系中对过氧化氢诱导的细胞毒性和基因毒性表现出最佳的细胞保护和基因保护活性：本研究证明了油橄榄和虎耳草单独或混合使用具有细胞保护和基因保护活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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