Non-human peptides revealed in blood reflect the composition of intestinal microbiota.

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-08-26 DOI:10.1186/s12915-024-01975-1
Georgij P Arapidi, Anatoly S Urban, Maria S Osetrova, Victoria O Shender, Ivan O Butenko, Olga N Bukato, Alexandr A Kuznetsov, Tatjana M Saveleva, Grigorii A Nos, Olga M Ivanova, Leonid V Lopukhov, Alexander V Laikov, Nina I Sharova, Margarita F Nikonova, Alexander N Mitin, Alexander I Martinov, Tatiana V Grigorieva, Elena N Ilina, Vadim T Ivanov, Vadim M Govorun
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Abstract

Background: The previously underestimated effects of commensal gut microbiota on the human body are increasingly being investigated using omics. The discovery of active molecules of interaction between the microbiota and the host may be an important step towards elucidating the mechanisms of symbiosis.

Results: Here, we show that in the bloodstream of healthy people, there are over 900 peptides that are fragments of proteins from microorganisms which naturally inhabit human biotopes, including the intestinal microbiota. Absolute quantitation by multiple reaction monitoring has confirmed the presence of bacterial peptides in the blood plasma and serum in the range of approximately 0.1 nM to 1 μM. The abundance of microbiota peptides reaches its maximum about 5 h after a meal. Most of the peptides correlate with the bacterial composition of the small intestine and are likely obtained by hydrolysis of membrane proteins with trypsin, chymotrypsin and pepsin - the main proteases of the gastrointestinal tract. The peptides have physicochemical properties that likely allow them to selectively pass the intestinal mucosal barrier and resist fibrinolysis.

Conclusions: The proposed approach to the identification of microbiota peptides in the blood, after additional validation, may be useful for determining the microbiota composition of hard-to-reach intestinal areas and monitoring the permeability of the intestinal mucosal barrier.

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血液中的非人类肽反映了肠道微生物群的组成。
背景:人们正越来越多地利用全息技术研究肠道微生物群对人体的影响。发现微生物群与宿主之间相互作用的活性分子可能是阐明共生机制的重要一步:结果:我们在这里发现,在健康人的血液中有 900 多种肽,它们是来自自然栖息于人类生物群(包括肠道微生物群)的微生物的蛋白质片段。通过多反应监测进行绝对定量,证实了血浆和血清中细菌肽的含量在 0.1 nM 至 1 μM 之间。餐后 5 小时左右,微生物群肽的含量达到最高。大多数肽与小肠的细菌组成相关,可能是通过胃肠道的主要蛋白酶--胰蛋白酶、糜蛋白酶和胃蛋白酶水解膜蛋白而获得的。这些肽的理化特性可能使它们有选择性地通过肠粘膜屏障并抵抗纤维蛋白溶解:结论:所提出的鉴定血液中微生物群肽的方法在经过进一步验证后,可用于确定难以到达的肠道区域的微生物群组成和监测肠粘膜屏障的通透性。
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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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