PmLBD3 links auxin and brassinosteroid signalling pathways on dwarfism in Prunus mume.

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-08-26 DOI:10.1186/s12915-024-01985-z
Yufan Ma, Chengdong Ma, Pengyu Zhou, Feng Gao, Wei Tan, Xiao Huang, Yang Bai, Minglu Li, Ziqi Wang, Faisal Hayat, Ting Shi, Zhaojun Ni, Zhihong Gao
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Abstract

Background: Grafting with dwarf rootstock is an efficient method to control plant height in fruit production. However, the molecular mechanism remains unclear. Our previous study showed that plants with Prunus mume (mume) rootstock exhibited a considerable reduction in plant height, internode length, and number of nodes compared with Prunus persica (peach) rootstock. The present study aimed to investigate the mechanism behind the regulation of plant height by mume rootstocks through transcriptomic and metabolomic analyses with two grafting combinations, 'Longyan/Mume' and 'Longyan/Peach'.

Results: There was a significant decrease in brassinolide levels in plants that were grafted onto mume rootstocks. Plant hormone signal transduction and brassinolide production metabolism gene expression also changed significantly. Flavonoid levels, amino acid and fatty acid metabolites, and energy metabolism in dwarf plants decreased. There was a notable upregulation of PmLBD3 gene expression in plant specimens that were subjected to grafting onto mume rootstocks. Auxin signalling cues promoted PmARF3 transcription, which directly controlled this upregulation. Through its binding to PmBAS1 and PmSAUR36a gene promoters, PmLBD3 promoted endogenous brassinolide inactivation and inhibited cell proliferation.

Conclusions: Auxin signalling and brassinolide levels are linked by PmLBD3. Our findings showed that PmLBD3 is a key transcription factor that regulates the balance of hormones through the auxin and brassinolide signalling pathways and causes dwarf plants in stone fruits.

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PmLBD3连接了辅助素和黄铜类固醇信号通路,对梅花矮化症有影响。
背景:在水果生产中,矮化砧木嫁接是控制植株高度的有效方法。然而,其分子机制仍不清楚。我们之前的研究表明,与桃砧木相比,使用梅砧木的植株在株高、节间长度和节数上都有明显的降低。本研究旨在通过对 "龙眼/梅 "和 "龙眼/桃 "两种嫁接组合的转录组和代谢组分析,研究梅砧木调节株高的机制:结果:嫁接到梅树砧木上的植株的黄铜内酯水平明显下降。植物激素信号转导和黄铜内酯生产代谢基因表达也发生了显著变化。矮化植株的类黄酮含量、氨基酸和脂肪酸代谢产物以及能量代谢均有所下降。在嫁接到梅花砧木上的植物标本中,PmLBD3 基因表达明显上调。叶绿素信号线索促进了 PmARF3 的转录,从而直接控制了这种上调。通过与 PmBAS1 和 PmSAUR36a 基因启动子结合,PmLBD3 促进了内源黄铜内酯失活并抑制了细胞增殖:结论:PmLBD3能促进内源黄铜内酯失活并抑制细胞增殖。我们的研究结果表明,PmLBD3是一个关键的转录因子,它通过辅助素和黄铜内酯信号途径调节激素平衡,并导致核果植株矮化。
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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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