Maciej Grzeszczuk, Monika Mrozowska, Alicja Kmiecik, Agnieszka Rusak, Karolina Jabłońska, Urszula Ciesielska, Piotr Dzięgiel, Katarzyna Nowińska
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引用次数: 0
Abstract
Background/aim: Cardiovascular diseases (CVD) are the leading cause of death worldwide. In 2019, 523 million people were diagnosed with CVD, with 18.6 million deaths. Improved treatment and diagnostics could reduce CVD's impact. Irisin (Ir) is crucial for heart function and may be a biomarker for heart attack. Ir is a glycoprotein with sugar residues attached to its protein structure. This glycosylation affects Ir stability, solubility, and receptor interactions on target cells. Its secondary structure includes a fibronectin type III domain, essential for its biological functions. Ir helps cardiomyocytes to respond to hypoxia and protects mitochondria. The aim of the study was to determine the FNDC5 gene expression level and the Ir level in HL-1 cardiomyocytes subjected to hypoxia.
Materials and methods: We examined the effect of hypoxia on the expression levels of the FNDC5 gene and those of Ir in mouse cardiomyocytes of the HL-1 cell line. Real-time PCR (RT-PCR) was used to estimate the expression levels of the FNDC5 gene. Western blot and immunofluorescence methods were used to analyze the Ir protein levels.
Results: Analyses showed an increased Ir level in HL-1 cardiomyocytes in response to hypoxia. This is the first study to confirm the presence of Ir in HL-1 cells.
Conclusion: The observed increase in Ir expression in murine cardiomyocytes is associated with the hypoxic environment and can be potentially used to diagnose hypoxia and CVD.
背景/目的:心血管疾病(CVD)是导致全球死亡的主要原因。2019 年,5.23 亿人被诊断患有心血管疾病,其中 1860 万人死亡。改善治疗和诊断可降低心血管疾病的影响。鸢尾素(Ir)对心脏功能至关重要,可能是心脏病发作的生物标志物。Ir是一种糖蛋白,其蛋白质结构上附有糖残基。这种糖基化作用会影响Ir的稳定性、溶解性以及在靶细胞上的受体相互作用。它的二级结构包括一个纤连蛋白 III 型结构域,这对其生物功能至关重要。Ir 能帮助心肌细胞应对缺氧并保护线粒体。本研究旨在确定缺氧条件下 HL-1 心肌细胞的 FNDC5 基因表达水平和 Ir 水平:我们研究了缺氧对 HL-1 细胞系小鼠心肌细胞中 FNDC5 基因表达水平和 Ir 表达水平的影响。实时 PCR(RT-PCR)用于估算 FNDC5 基因的表达水平。采用 Western 印迹和免疫荧光方法分析 Ir 蛋白水平:结果:分析表明,缺氧时 HL-1 心肌细胞中的 Ir 含量增加。这是首次证实 HL-1 细胞中存在 Ir 的研究:结论:在小鼠心肌细胞中观察到的 Ir 表达增加与缺氧环境有关,可用于诊断缺氧和心血管疾病。
期刊介绍:
IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management.
The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.