Photobiomodulation Effect of Diode Laser on Differentiation of Osteoprogenitor Cells in Rat Bone Marrow.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-09-01 DOI:10.21873/invivo.13685

Eisuke Iso, Takahide Yamazaki, Norika Kobayashi, Hiroshi Kadokura, Takako Tsuchiya, Yuka Kato, Satoshi Yokose

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Abstract

Background/aim: Bone marrow cells contain nonhematopoietic cells with the ability to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Mechanical stress influences osteoblast differentiation of bone marrow cells into osteogenic, chondrogenic, and adipogenic lineages, measurable as the abundance of alkaline phosphatase-positive (ALP⁺) colony-forming unit-fibroblasts (CFU-F); however, the effect of diode laser irradiation on osteoblast differentiation is unknown. The aim of this study was to analyze the effects of photobiomodulation on the osteogenic differentiation of mesenchymal stem cells in the bone marrow, using the CFU-F assay.

Materials and methods: Bone marrow cells isolated from rat tibiae were cultured and irradiated with a diode laser (wavelength 808 nm) at a total energy of 0 J (control), 50 J, and 150 J.

Results: On day 7 after irradiation, ALP⁺ CFU-F were most abundant in the 50 J group and the least abundant in the 150 J group. Mineralized nodule formation was observed after long-term culture (21 days). Compared with the control group, there were significantly more nodules in the 50 J group and significantly fewer nodules in the 150 J group. Osteocalcin mRNA expression was highest in the 50 J group, and there was no difference between the control and 150 J groups.

Conclusion: Irradiation with 50 J was effective in stimulating osteogenesis in bone marrow stem cells. These findings suggest that diode laser irradiation can induce osteogenesis in rat bone marrow cells in an energy-dependent manner, and appears suitable for application in bone regeneration therapy.

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二极管激光对大鼠骨髓中成骨细胞分化的光生物调节作用

背景/目的：骨髓细胞含有非造血细胞，具有分化为成骨、软骨和成脂系的能力。机械应力会影响骨髓细胞向成骨、软骨和成脂系分化，可通过碱性磷酸酶阳性（ALP+）集落形成单位-成纤维细胞（CFU-F）的丰度来测量；然而，二极管激光照射对成骨细胞分化的影响尚不清楚。本研究旨在利用 CFU-F 试验分析光生物调制对骨髓间充质干细胞成骨分化的影响：从大鼠胫骨分离的骨髓细胞经培养后用二极管激光器（波长808 nm）照射，总能量分别为0 J（对照组）、50 J和150 J：结果：辐照后第 7 天，50 J 组的 ALP+ CFU-F 最多，150 J 组最少。长期培养（21 天）后观察到矿化结核形成。与对照组相比，50 J 组的结节明显增多，150 J 组的结节明显减少。50 J 组的骨钙素 mRNA 表达量最高，对照组和 150 J 组之间没有差异：结论：50 焦耳的辐照能有效刺激骨髓干细胞的成骨。这些研究结果表明，二极管激光辐照能以能量依赖的方式诱导大鼠骨髓细胞成骨，似乎适合应用于骨再生治疗。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.