Separation of astragaloside IV from Astragalus membranaceus based on high-speed countercurrent chromatography in continuous injection mode.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS Phytochemical Analysis Pub Date : 2024-08-26 DOI:10.1002/pca.3438
Yin-Di Zhao, Qian-Xia Su, Ning-Li Wang, Dong Pei, Xin-Yi Huang
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Abstract

Introduction: Astragaloside IV (AS-IV) is an index for the quality evaluation of the traditional Chinese medicine Astragalus and an important material basis for Astragalus to exert its medicinal effects, and it is difficult to obtain a single AS-IV by ordinary separation methods.

Objective: To find a new isolation method that can prepare AS-IV quickly and efficiently.

Methodology: AS-IV was isolated from Astragalus membranaceus extract by high-speed countercurrent chromatography using a two-phase solvent system consisting of ethyl acetate/n-butanol/water (4.2:0.8:5, v/v) at a speed of 950 rpm at a flow rate of 2 mL/min using one of the high-speed countercurrent chromatographic sequential injection models developed during the previous study.

Results: Compared with the common countercurrent chromatographic separation, this separation method increased the injection volume and yield by 4-fold and 4.47-fold, respectively, with only about 1.2-fold increase in solvent consumption and separation time, and the purity was basically not reduced, and 55.9 mg of AS-IV, with a purity of 96.95%, was finally prepared from 400 mg of the crude extract in 240 min.

Conclusion: The continuous injection mode of high-speed countercurrent chromatography was able to successfully prepare a large amount of AS-IV with high purity at one time.

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基于高速逆流色谱连续进样模式分离黄芪中的黄芪皂苷 IV
引言黄芪皂苷IV(AS-IV)是评价中药黄芪质量的一项指标,也是黄芪发挥药效的重要物质基础,而普通的分离方法很难获得单一的AS-IV:方法:从黄芪中分离出 AS-IV:从黄芪提取物中采用高速逆流色谱法分离AS-IV,使用的溶剂系统为乙酸乙酯/正丁醇/水(4.2:0.8:5, v/v),流速为2 mL/min,转速为950 rpm:结果:与普通逆流色谱分离相比,该分离方法的进样量和收率分别提高了4倍和4.47倍,而溶剂消耗量和分离时间仅增加了约1.2倍,纯度基本没有降低,400 mg粗提物在240 min内最终制备出55.9 mg AS-IV,纯度为96.95%:结论:高速逆流色谱的连续进样模式能够一次性成功制备大量高纯度的 AS-IV。
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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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