High-performance liquid chromatography coupled to Orbitrap mass spectrometry for screening of common new psychoactive substances and other drugs in biological samples

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL Journal of forensic sciences Pub Date : 2024-08-26 DOI:10.1111/1556-4029.15607
Manqing Nie BSc, Tianai Zhang BSc, Xuan Wang BSc, Xuan Zhao MM, Chunying Luo PhD, Lian Wang PhD, Xiaoli Zou PhD
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Abstract

The complexity of the drug market and the constant updating of drugs have been challenging issues for drug regulatory authorities. With the emergence of new psychoactive substances (NPS) and the nonmedical use of prescription drugs, forensic and toxicology laboratories have had to adopt new drug screening methods and advanced instrumentation. Using high-performance liquid chromatography coupled with Orbitrap mass spectrometry, we developed a screening method for common NPS and other drugs. Two milliliters of mixed solvent of n-hexane and ethyl acetate (1:1, v:v) were added to 500 μL of blood or urine sample for liquid–liquid extraction, and methanol extraction was used for hair samples. The developed method was applied to 3897 samples (including 332 blood samples, 885 urine samples, and 2680 hair samples) taken from drug addicts in a province of China during 2019–2021. For urine and blood samples, the limits of detection (LODs) ranged from 1.68 pg/mL to 10.7 ng/mL. For hair samples, the LODs ranged from 3.30 × 10−5 to 4.21 × 10−3 ng/mg. The matrix effects of urine, blood, and hair samples were in the range of 47.6%–121%, 39.8%–139%, and 6.35%–118%, respectively. And the intra-day precision was 3.5%–6.0% and the inter-day precision was 4.18%–9.90%. Analysis of the actual samples showed an overall positive detection rate of 58.9%, with 5.32% of the samples indicating the use of multiple drugs.

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高效液相色谱法与 Orbitrap 质谱联用技术用于筛查生物样本中常见的新型精神活性物质和其他药物。
药物市场的复杂性和药物的不断更新一直是药物监管当局面临的挑战性问题。随着新精神活性物质(NPS)和处方药的非医疗使用的出现,法医和毒理学实验室不得不采用新的药物筛选方法和先进的仪器。利用高效液相色谱法和 Orbitrap 质谱法,我们开发出了一种常见 NPS 和其他药物的筛查方法。在 500 μL 血液或尿液样本中加入两毫升正己烷和乙酸乙酯(1:1,v:v)混合溶剂进行液-液萃取,毛发样本采用甲醇萃取。所建立的方法适用于2019-2021年期间在中国某省采集的3897份吸毒者样本(包括332份血液样本、885份尿液样本和2680份毛发样本)。尿样和血样的检出限为 1.68 pg/mL 至 10.7 ng/mL。头发样本的检出限为 3.30 × 10-5 至 4.21 × 10-3 纳克/毫克。尿液、血液和毛发样品的基质效应分别为 47.6%-121%、39.8%-139% 和 6.35%-118%。日内精密度为 3.5%-6.0%,日间精密度为 4.18%-9.90%。对实际样本的分析表明,总体阳性检出率为 58.9%,其中 5.32% 的样本表明使用了多种药物。
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来源期刊
Journal of forensic sciences
Journal of forensic sciences 医学-医学:法
CiteScore
4.00
自引率
12.50%
发文量
215
审稿时长
2 months
期刊介绍: The Journal of Forensic Sciences (JFS) is the official publication of the American Academy of Forensic Sciences (AAFS). It is devoted to the publication of original investigations, observations, scholarly inquiries and reviews in various branches of the forensic sciences. These include anthropology, criminalistics, digital and multimedia sciences, engineering and applied sciences, pathology/biology, psychiatry and behavioral science, jurisprudence, odontology, questioned documents, and toxicology. Similar submissions dealing with forensic aspects of other sciences and the social sciences are also accepted, as are submissions dealing with scientifically sound emerging science disciplines. The content and/or views expressed in the JFS are not necessarily those of the AAFS, the JFS Editorial Board, the organizations with which authors are affiliated, or the publisher of JFS. All manuscript submissions are double-blind peer-reviewed.
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