Early fasting does not impact gonadal size nor vasa gene expression in the European seabass Dicentrarchus labrax.

Abstract

Primordial germ cells (PGCs) play a crucial role in sexual development in fish, with recent studies revealing their influence on sexual fate. Notably, PGC number at specific developmental stages can determine whether an individual develops as male or female. Temperature was shown to impact PGC proliferation and the subsequent phenotypic sex in some fish species. Here, we aimed at testing the role of food deprivation on gonad development in the European seabass Dicentrarchus labrax, a species displaying a polygenic sex determination system with an environmental influence. We subjected larvae to two periods of starvation to investigate whether restricting growth affects both gonadal size and vasa gene expression. We first confirmed by immunohistochemistry that Vasa was indeed a marker of PGCs in the European seabass, as in other fish species. We also showed that vasa correlated positively with fish size, confirming that it could be used as a marker of feminization. However, starvation did not show any significant effects on vasa expression nor on gonadal size. It is hypothesized that evolutionary mechanisms likely safeguard PGCs against environmental stressors to ensure reproductive success. Further research is needed to elucidate the intricate interplay between environmental cues, PGC biology, and sexual differentiation in fish.