Cancer cell states: Lessons from ten years of single-cell RNA-sequencing of human tumors

IF 48.8 1区 医学 Q1 CELL BIOLOGY Cancer Cell Pub Date : 2024-08-29 DOI:10.1016/j.ccell.2024.08.005
Itay Tirosh, Mario L. Suva
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Abstract

Human tumors are intricate ecosystems composed of diverse genetic clones and malignant cell states that evolve in a complex tumor micro-environment. Single-cell RNA-sequencing (scRNA-seq) provides a compelling strategy to dissect this intricate biology and has enabled a revolution in our ability to understand tumor biology over the last ten years. Here we reflect on this first decade of scRNA-seq in human tumors and highlight some of the powerful insights gleaned from these studies. We first focus on computational approaches for robustly defining cancer cell states and their diversity and highlight some of the most common patterns of gene expression intra-tumor heterogeneity (eITH) observed across cancer types. We then discuss ambiguities in the field in defining and naming such eITH programs. Finally, we highlight critical developments that will facilitate future research and the broader implementation of these technologies in clinical settings.

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癌细胞状态:人类肿瘤单细胞 RNA 测序十年的启示
人类肿瘤是一个错综复杂的生态系统,由不同的基因克隆和恶性细胞状态组成,在复杂的肿瘤微环境中不断演变。单细胞 RNA 测序(scRNA-seq)提供了一种令人信服的策略来剖析这种错综复杂的生物学特性,并在过去十年中使我们了解肿瘤生物学的能力发生了革命性的变化。在此,我们回顾了人类肿瘤 scRNA-seq 研究的第一个十年,并重点介绍了从这些研究中获得的一些重要启示。首先,我们将重点放在稳健定义癌细胞状态及其多样性的计算方法上,并强调在不同癌症类型中观察到的一些最常见的基因表达肿瘤内异质性(eITH)模式。然后,我们讨论了该领域在定义和命名此类 eITH 方案时存在的模糊之处。最后,我们强调了将促进未来研究和在临床环境中更广泛实施这些技术的关键发展。
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来源期刊
Cancer Cell
Cancer Cell 医学-肿瘤学
CiteScore
55.20
自引率
1.20%
发文量
179
审稿时长
4-8 weeks
期刊介绍: Cancer Cell is a journal that focuses on promoting major advances in cancer research and oncology. The primary criteria for considering manuscripts are as follows: Major advances: Manuscripts should provide significant advancements in answering important questions related to naturally occurring cancers. Translational research: The journal welcomes translational research, which involves the application of basic scientific findings to human health and clinical practice. Clinical investigations: Cancer Cell is interested in publishing clinical investigations that contribute to establishing new paradigms in the treatment, diagnosis, or prevention of cancers. Insights into cancer biology: The journal values clinical investigations that provide important insights into cancer biology beyond what has been revealed by preclinical studies. Mechanism-based proof-of-principle studies: Cancer Cell encourages the publication of mechanism-based proof-of-principle clinical studies, which demonstrate the feasibility of a specific therapeutic approach or diagnostic test.
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